Journal ArticleDOI
Glucose oxidase-magnetite nanoparticle bioconjugate for glucose sensing
TLDR
Preparing and functionalization of magnetite (Fe3O4) nanoparticles 20 nm in diameter and the successful covalent conjugation of the enzyme glucose oxidase to the amino-modified nanoparticle surface are presented.Abstract:
Immobilization of bioactive molecules on the surface of magnetic nanoparticles is of great interest, because the magnetic properties of these bioconjugates promise to greatly improve the delivery and recovery of biomolecules in biomedical applications. Here we present the preparation and functionalization of magnetite (Fe3O4) nanoparticles 20 nm in diameter and the successful covalent conjugation of the enzyme glucose oxidase to the amino-modified nanoparticle surface. Functionalization of the magnetic nanoparticle surface with amino groups greatly increased the amount and activity of the immobilized enzyme compared with immobilization procedures involving physical adsorption. The enzymatic activity of the glucose oxidase-coated magnetic nanoparticles was investigated by monitoring oxygen consumption during the enzymatic oxidation of glucose using a ruthenium phenanthroline fluorescent complex for oxygen sensing. The glucose oxidase-coated magnetite nanoparticles could function as nanometric glucose sensors in glucose solutions of concentrations up to 20 mmol L−1. Immobilization of glucose oxidase on the nanoparticles also increased the stability of the enzyme. When stored at 4°C the nanoparticle suspensions maintained their bioactivity for up to 3 months.read more
Citations
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Journal ArticleDOI
Magnetic nanoparticles: design and characterization, toxicity and biocompatibility, pharmaceutical and biomedical applications.
TL;DR: Biocompatibility, Pharmaceutical and Biomedical Applications L. Harivardhan Reddy,‡ Jose ́ L. Arias, Julien Nicolas,† and Patrick Couvreur*,†.
Journal ArticleDOI
Potential applications of enzymes immobilized on/in nano materials: A review
TL;DR: N nanoparticle-based immobilization of enzymes showed a broader working pH and temperature range and higher thermal stability than the native enzymes, and it is possible that co-immobilization of multi-enzymes could be achieved on these nanoparticles.
Journal ArticleDOI
Optical methods for sensing glucose
TL;DR: This critical review covers the present state of the art in optical sensing of glucose using methods based on monitoring the optical properties of intrinsically fluorescent or labeled enzymes, their co-enzymes and co-substrates.
Journal ArticleDOI
Iron oxide nanoparticles–chitosan composite based glucose biosensor
Ajeet Kaushik,Raju Khan,Pratima R. Solanki,Pratibha Pandey,Javed Alam,Sharif Ahmad,Bansi D. Malhotra +6 more
TL;DR: The GOx/CH-Fe(3)O(4)/ITO nanocomposite bioelectrode has response time of 5s, linearity as 10-400 mgdL(-1) of glucose, sensitivity as 9.3 microA/(mgdLcm(2)) and shelf life of about 8 weeks under refrigerated conditions.
Journal ArticleDOI
Conductive polymer-based sensors for biomedical applications
Shruti Nambiar,John T.W. Yeow +1 more
TL;DR: This paper focuses onCP-based sensor elements and the state-of-art of CP-based sensing devices that have potential applications as tools in clinical diagnosis and surgical interventions and some of the key issues related to CPs are highlighted.
References
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Journal ArticleDOI
TOPICAL REVIEW: Applications of magnetic nanoparticles in biomedicine
Book ChapterDOI
Applications of magnetic nanoparticles in biomedicine
TL;DR: The physical principles underlying some current biomedical applications of magnetic nanoparticles are reviewed and the relevant physics of magnetic materials and their responses to applied magnetic fields are surveyed.
Journal ArticleDOI
Size-Controlled Synthesis of Magnetite Nanoparticles
Shouheng Sun,Hao Zeng +1 more
TL;DR: The reported procedure can be used as a general approach to various ferrite nanoparticles and nanoparticle superlattices.
Journal ArticleDOI
Synthesis of highly crystalline and monodisperse maghemite nanocrystallites without a size-selection process.
TL;DR: High-temperature (300 degrees C) aging of iron-oleic acid metal complex, which was prepared by the thermal decomposition of iron pentacarbonyl in the presence of oleic acid at 100 degrees C, was found to generate monodisperse iron nanoparticles.
Journal ArticleDOI
The preparation of magnetic nanoparticles for applications in biomedicine
Pedro Tartaj,María del Puerto Morales,Sabino Veintemillas-Verdaguer,T. González-Carreño,Carlos J. Serna +4 more
TL;DR: In this article, a review of state-of-the-art synthetic routes for the preparation of magnetic nanoparticles useful for biomedical applications is presented, with a special emphasis on showing the benefits of using nanoparticles.
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