Growth factors and membrane depolarization activate distinct programs of early response gene expression: dissociation of fos and jun induction.
TLDR
It is concluded that multiple nonconvergent signal transduction pathways control early response gene expression and that the diversity and specificity of cellular response to environmental change can be accounted for by the differential combinatorial induction of a relatively small number of early response genes.Abstract:
A set of early response genes has been identified whose transcription in fibroblasts is rapidly induced in response to growth factors. Prototype members of this group, c-fos and c-jun, encode products that form a heterodimer and have been implicated in the regulation of gene expression and cell growth. It is thought that other early response genes also encode critical mediators of the cell's response to external stimuli. We have used PC12 pheochromocytoma cells as a model system to test the hypothesis that different extracellular signals induce distinct patterns of expression of early response genes. Our results indicate that membrane depolarization, induced either by potassium chloride or by the neurotransmitter analog nicotine, activates a program of gene expression distinct from that activated by nerve growth factor or epidermal growth factor. Notably, c-fos and c-jun activation can be dissociated; whereas c-jun is coinduced with c-fos and jun-B after growth factor stimulation, membrane depolarization activates c-fos and jun-B without stimulating c-jun. Fos may therefore form transcription complexes with alternative cofactors under different stimulation conditions. nur/77 and zif/268, which encode putative transcription factors, also show markedly different responses to growth factors and depolarization. We conclude that multiple nonconvergent signal transduction pathways control early response gene expression. Our findings also indicate that the diversity and specificity of cellular response to environmental change can be accounted for by the differential combinatorial induction of a relatively small number of early response genes.read more
Citations
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NID67, a small putative membrane protein, is preferentially induced by NGF in PC12 pheochromocytoma cells.
TL;DR: The cloning of a previously unknown primary response gene, NID67, is reported, which has a robust induction by NGF and FGF, both of which cause PC12 cells to differentiate and may play a similar role in cellular physiology.
Journal ArticleDOI
Growth factors and growth control
TL;DR: A number of oncogenes have been found to encode mutated forms of growth factors, growth factor receptors, proteins involved in signal transduction, and proteins encoded by growth-factor-inducible genes that support the notion that the biochemical consequences of growth factor-receptor interactions are key events in cell growth control.
Journal ArticleDOI
Growth conditions differentially affect the constitutive expression of primary response genes in cultured cereballar granule cells
Agata Copani,Valeria Bruno,P. Dell'Albani,Giuseppe Battaglia,Vincenza Barresi,Alessandra Caruso,Ferdinando Nicoletti,Ferdinando Nicoletti,Daniele F. Condorelli +8 more
TL;DR: It is speculated that changes in the expression of zif/268 are important in the gene program associated with the induction of apoptosis by trophic deprivation in cultured neurons.
Journal ArticleDOI
A calcineurin-mediated scaling mechanism that controls a K+-leak channel to regulate morphogen and growth factor transcription.
Chao Yi,Chao Yi,Tim Wgm Spitters,Ezz Al-Din Ahmed Al-Far,Sen Wang,Sen Wang,Tian Long Xiong,Tian Long Xiong,Simian Cai,Xin Yan,Kaomei Guan,Michael Wagner,Ali El-Armouche,Christopher L. Antos,Christopher L. Antos +14 more
TL;DR: Yi et al. as discussed by the authors used genetically modified zebrafish to investigate how the Kcnk5b channel could control genes responsible for appendage growth and showed that calcineurin can modify the activity of proteins to control its activity.
Patent
Human neuronal nicotinic acetylcholine receptor and cells transformed with same DNA and mRNA encoding an--subunit of
TL;DR: In this paper, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically α4 and α7) and β subunits(specifically β4) are provided.
References
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Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements
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