Journal ArticleDOI
Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering
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TLDR
The SEM and immunocytochemical analysis showed that the aligned PG scaffold greatly promoted cell attachment and alignment because of the biological components and ordered topography of the scaffolds, which concluded that thealigned PG nanofibrous scaffolds could be more promising substrates suitable for the regeneration of infarct myocardium and other cardiac defects.Abstract:
Cardiac tissue engineering (TE) is one of the most promising strategies to reconstruct the infarct myocardium and the major challenge involves producing a bioactive scaffold with anisotropic properties that assist in cell guidance to mimic the heart tissue. In this study, random and aligned poly(e-caprolactone)/gelatin (PG) composite nanofibrous scaffolds were electrospun to structurally mimic the oriented extracellular matrix (ECM). Morphological, chemical and mechanical properties of the electrospun PG nanofibers were evaluated by scanning electron microscopy (SEM), water contact angle, attenuated total reflectance Fourier transform infrared spectroscopy and tensile measurements. Results indicated that PG nanofibrous scaffolds possessed smaller fiber diameters (239 ± 37 nm for random fibers and 269 ± 33 nm for aligned fibers), increased hydrophilicity, and lower stiffness compared to electrospun PCL nanofibers. The aligned PG nanofibers showed anisotropic wetting characteristics and mechanical properties, which closely match the requirements of native cardiac anisotropy. Rabbit cardiomyocytes were cultured on electrospun random and aligned nanofibers to assess the biocompatibility of scaffolds, together with its potential for cell guidance. The SEM and immunocytochemical analysis showed that the aligned PG scaffold greatly promoted cell attachment and alignment because of the biological components and ordered topography of the scaffolds. Moreover, we concluded that the aligned PG nanofibrous scaffolds could be more promising substrates suitable for the regeneration of infarct myocardium and other cardiac defects. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.read more
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Journal ArticleDOI
PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues
Mahshid Kharaziha,Mehdi Nikkhah,Mehdi Nikkhah,Su Ryon Shin,Su Ryon Shin,Su Ryon Shin,Nasim Annabi,Nasim Annabi,Nasim Annabi,Nafiseh Masoumi,Nafiseh Masoumi,Akhilesh K. Gaharwar,Gulden Camci-Unal,Gulden Camci-Unal,Ali Khademhosseini,Ali Khademhosseini,Ali Khademhosseini +16 more
TL;DR: This study suggests that the aligned nanofibrous PGS:gelatin scaffold support cardiac cell organization, phenotype and contraction and could potentially be used to develop clinically relevant constructs for cardiac tissue engineering.
Journal ArticleDOI
Tough and Flexible CNT-Polymeric Hybrid Scaffolds for Engineering Cardiac Constructs
Mahshid Kharaziha,Su Ryon Shin,Mehdi Nikkhah,Seda Nur Topkaya,Nafiseh Masoumi,Nasim Annabi,Mehmet R. Dokmeci,Ali Khademhosseini +7 more
TL;DR: Tough and flexible hybrid scaffolds with enhanced electrical properties composed of carbon nanotubes (CNTs) embedded aligned poly(glycerol sebacate):gelatin (PG) electrospun nanofibers with superior mechanical properties with enhanced CM beating properties are developed.
Journal ArticleDOI
Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering.
TL;DR: The desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic.
Journal ArticleDOI
Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications.
Dan Kai,Molamma P. Prabhakaran,Benjamin Stahl,Benjamin Stahl,Markus Eblenkamp,Erich Wintermantel,Seeram Ramakrishna +6 more
TL;DR: Hydrogel-based biomaterial systems have great potential for tissue reconstruction by serving as temporary scaffolds and cell delivery vehicles for tissue engineering (TE), and the 'nanocomposite hydrogels' might provide the necessary mechanical support and could be promising cell delivery systems for tissue regeneration.
Journal ArticleDOI
Electrospinning of poly(glycerol sebacate)-based nanofibers for nerve tissue engineering.
Jue Hu,Dan Kai,Hongye Ye,Lingling Tian,Xin Ding,Seeram Ramakrishna,Xian Jun Loh,Xian Jun Loh +7 more
TL;DR: The synthesis of PGS-based copolymers with methyl methacrylate might be a promising approach to enhance their processability, and therefore advancing bioscaffold engineering for various TE applications.
References
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Electrospinning of polymeric nanofibers for tissue engineering applications: a review.
TL;DR: Electrospinning is examined by providing a brief description of the theory behind the process, examining the effect of changing the process parameters on fiber morphology, and discussing the potential applications and impacts of electrospinning on the field of tissue engineering.
Journal ArticleDOI
Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering.
TL;DR: The aligned nanofibrous PLLA scaffold could be used as a potential cell carrier in neural tissue engineering after being evaluated in vitro using neural stem cells as a model cell line.
Journal ArticleDOI
Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering.
Laleh Ghasemi-Mobarakeh,Molamma P. Prabhakaran,Mohammad Morshed,Mohammad Hossein Nasr-Esfahani,Seeram Ramakrishna +4 more
TL;DR: PCL/gelatin 70:30 nanofiber was found to exhibit the most balanced properties to meet all the required specifications for nerve tissue and was used for in vitro culture of nerve stem cells and proved to be a promising biomaterial suitable for nerve regeneration.
Journal ArticleDOI
Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution.
E J Chong,Toan Thang Phan,Ivor J. Lim,Yanzhong Zhang,Boon-Huat Bay,Seeram Ramakrishna,Chwee Teck Lim +6 more
TL;DR: This study proposes a cost-effective composite consisting of a nanofibrous scaffold directly electrospun onto a polyurethane dressing (Tegaderm, 3M Medical) - which it is called the Tegaderm-nanofiber (TG-NF) construct - for dermal wound healing.
Journal ArticleDOI
Accordion-like honeycombs for tissue engineering of cardiac anisotropy.
George C. Engelmayr,Mingyu Cheng,Christopher J. Bettinger,Jeffrey T. Borenstein,Robert Langer,Lisa E. Freed +5 more
TL;DR: Accordion-like honeycombs can overcome principal structural-mechanical limitations of previous scaffolds, promoting the formation of grafts with aligned heart cells and mechanical properties more closely resembling native myocardium.