H2S Signals Through Protein S-Sulfhydration
Asif K. Mustafa,Moataz M. Gadalla,Nilkantha Sen,Seyun Kim,Weitong Mu,Sadia K. Gazi,Roxanne K. Barrow,Guangdong Yang,Rui Wang,Solomon H. Snyder +9 more
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TLDR
Ex vivo endogenous H2S physiologically modifies cysteine residues in many proteins, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin, converting Cysteine -SH groups to -SSH groups in a process the authors call S-sulfhydration.Abstract:
Hydrogen sulfide (H2S), a messenger molecule generated by cystathionine gamma-lyase, acts as a physiologic vasorelaxant. Mechanisms whereby H2S signals have been elusive. We now show that H2S physiologically modifies cysteines in a large number of proteins by S-sulfhydration. About 10 to 25% of many liver proteins, including actin, tubulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are sulfhydrated under physiological conditions. Sulfhydration augments GAPDH activity and enhances actin polymerization. Sulfhydration thus appears to be a physiologic posttranslational modification for proteins.read more
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The coordination of S-sulfhydration, S-nitrosylation, and phosphorylation of endothelial nitric oxide synthase by hydrogen sulfide.
TL;DR: The data suggest that H2S increases eNOS activity by inducing the S-sulfhydration of eN OS, promoting its phosphorylation, inhibiting its S-nitrosylation, and increasing ENOS dimerization, whereas NO decreases eNos activity by promoting the formation of e NOS monomers.
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Mechanistic Insights into the H2S-Mediated Reduction of Aryl Azides Commonly Used in H2S Detection
TL;DR: It is established that HS(-, rather than diprotic H2S, is the active species required for aryl azide reduction, and mechanistic investigations support that this intermediate is attacked by HS(-) in the rate-limiting step of the reduction reaction.
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Regulators of the transsulfuration pathway
TL;DR: This review focuses on the regulation of the transsulfuration pathway pertaining to cysteine and H2S, which could be targeted to develop novel therapeutics.
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Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain
Yuka Kimura,Yukiko Toyofuku,Shin Koike,Norihiro Shibuya,Noriyuki Nagahara,David J. Lefer,Yuki Ogasawara,Hideo Kimura +7 more
TL;DR: New insights into the physiology of H1S3 and H2S are provided, as well as novel therapeutic targets for diseases in which these molecules are involved, are provided.
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Cystathionine γ-Lyase Protects against Renal Ischemia/Reperfusion by Modulating Oxidative Stress
Eelke M. Bos,Rui Wang,Pauline M. Snijder,Miriam Boersema,Jeffrey Damman,Ming Fu,Jill Moser,Jan-Luuk Hillebrands,Rutger J. Ploeg,Guangdong Yang,Henri G. D. Leuvenink,Harry van Goor +11 more
TL;DR: Treatment with exogenous H2S rescued CSE knockout mice from the injury and mortality associated with renal ischemia, and overexpression of CSE in vitro reduced the amount of reactive oxygen species produced during stress.
References
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Journal ArticleDOI
H2S as a Physiologic Vasorelaxant: Hypertension in Mice with Deletion of Cystathionine γ-Lyase
Guangdong Yang,Guangdong Yang,Lingyun Wu,Bo Jiang,Wei Yang,Jiansong Qi,Kun Cao,Qinghe Meng,Asif K. Mustafa,Weitong Mu,Shengming Zhang,Solomon H. Snyder,Rui Wang,Rui Wang +13 more
TL;DR: It is shown that H2S is physiologically generated by cystathionine γ-lyase (CSE) and that genetic deletion of this enzyme in mice markedly reduces H 2S levels in the serum, heart, aorta, and other tissues.
Journal ArticleDOI
Protein S-nitrosylation: purview and parameters.
Douglas T. Hess,Akio Matsumoto,Sung Oog Kim,Harvey E. Marshall,Jonathan S. Stamler,Jonathan S. Stamler +5 more
TL;DR: S-nitrosylation conveys a large part of the ubiquitous influence of nitric oxide on cellular signal transduction, and provides a mechanism for redox-based physiological regulation.
Journal ArticleDOI
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
TL;DR: It is demonstrated that H2S is an important endogenous vasoactive factor and the first identified gaseous opener of KATP channels in vascular SMCs and production from vascular tissues was enhanced by nitric oxide.
Journal ArticleDOI
Hydrogen sulphide and its therapeutic potential
TL;DR: The physiology and biochemistry of H2S is overviews, the effects of H 2S inhibitors or H2s donors in animal models of disease are summarized, the potential options for the therapeutic exploitation of H1S are outlined and they are outlined.
Journal ArticleDOI
Protein S-nitrosylation: a physiological signal for neuronal nitric oxide.
Samie R. Jaffrey,Hediye Erdjument-Bromage,Christopher D. Ferris,Paul Tempst,Solomon H. Snyder +4 more
TL;DR: Protein S-nitrosylation is established as a physiological signalling mechanism for neuronally generated NO in mice harbouring a genomic deletion of neuronal NO synthase (nNOS).
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