H2S Signals Through Protein S-Sulfhydration
Asif K. Mustafa,Moataz M. Gadalla,Nilkantha Sen,Seyun Kim,Weitong Mu,Sadia K. Gazi,Roxanne K. Barrow,Guangdong Yang,Rui Wang,Solomon H. Snyder +9 more
Reads0
Chats0
TLDR
Ex vivo endogenous H2S physiologically modifies cysteine residues in many proteins, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin, converting Cysteine -SH groups to -SSH groups in a process the authors call S-sulfhydration.Abstract:
Hydrogen sulfide (H2S), a messenger molecule generated by cystathionine gamma-lyase, acts as a physiologic vasorelaxant. Mechanisms whereby H2S signals have been elusive. We now show that H2S physiologically modifies cysteines in a large number of proteins by S-sulfhydration. About 10 to 25% of many liver proteins, including actin, tubulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are sulfhydrated under physiological conditions. Sulfhydration augments GAPDH activity and enhances actin polymerization. Sulfhydration thus appears to be a physiologic posttranslational modification for proteins.read more
Citations
More filters
Journal ArticleDOI
Hydrogen sulfide signaling in regulation of cell behaviors.
TL;DR: A better understanding of the regulation of H2S on cell behavior change and the underlying molecular mechanisms will help to develop novel and more effective strategies for clinical therapy.
Journal ArticleDOI
A comparison of the chemical biology of hydropersulfides (RSSH) with other protective biological antioxidants and nucleophiles
TL;DR: The hydropersulfide (RSSH) functional group has received significant recent interest due to its unique chemical properties that set it apart from other biological species, and the inherent chemical properties of RSSH versus these other protectants will be discussed and contrasted.
Journal ArticleDOI
H2S Relaxes Vas Deferens Smooth Muscle by Modulating the Large Conductance Ca2+‐Activated K+ (BKCa) Channels via a Redox Mechanism
TL;DR: It is concluded that H(2) S relaxed the VD smooth muscle by targeting BK(Ca) channels via redox-mediated mechanism.
Journal ArticleDOI
Hydrogen sulfide preserves endothelial nitric oxide synthase function by inhibiting proline-rich kinase 2: Implications for cardiomyocyte survival and cardioprotection
Sofia Iris Bibli,Sofia Iris Bibli,Csaba Szabó,Athanasia Chatzianastasiou,Bert Luck,Sven Zukunft,Ingrid Fleming,Andreas Papapetropoulos,Andreas Papapetropoulos +8 more
TL;DR: It is concluded that H2S relieves the inhibitory effect of PYK2 on eNOS, allowing the latter to produce greater amounts of NO, thereby affording cardioprotection.
Journal ArticleDOI
Reprogrammed transsulfuration promotes basal-like breast tumor progression via realigning cellular cysteine persulfidation.
Katalin Erdelyi,Tamás Ditrói,Henrik J. Johansson,Ágnes Czikora,Noémi Balog,Laxmi Silwal-Pandit,Tomoaki Ida,Judit Olasz,Dorottya Hajdú,Zoltán Mátrai,Orsolya Csuka,Koji Uchida,József Tóvári,Olav Engebråten,Takaaki Akaike,Anne-Lise Børresen Dale,Miklós Kásler,Janne Lehtiö,Péter Nagy +18 more
TL;DR: In this paper, the authors showed that CBS silencing made BLBC cells less invasive, proliferate slower, more vulnerable to oxidative stress and cystine deprivation, prone to ferroptosis, and less responsive to HIF1α activation under hypoxia.
References
More filters
Journal ArticleDOI
H2S as a Physiologic Vasorelaxant: Hypertension in Mice with Deletion of Cystathionine γ-Lyase
Guangdong Yang,Guangdong Yang,Lingyun Wu,Bo Jiang,Wei Yang,Jiansong Qi,Kun Cao,Qinghe Meng,Asif K. Mustafa,Weitong Mu,Shengming Zhang,Solomon H. Snyder,Rui Wang,Rui Wang +13 more
TL;DR: It is shown that H2S is physiologically generated by cystathionine γ-lyase (CSE) and that genetic deletion of this enzyme in mice markedly reduces H 2S levels in the serum, heart, aorta, and other tissues.
Journal ArticleDOI
Protein S-nitrosylation: purview and parameters.
Douglas T. Hess,Akio Matsumoto,Sung Oog Kim,Harvey E. Marshall,Jonathan S. Stamler,Jonathan S. Stamler +5 more
TL;DR: S-nitrosylation conveys a large part of the ubiquitous influence of nitric oxide on cellular signal transduction, and provides a mechanism for redox-based physiological regulation.
Journal ArticleDOI
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
TL;DR: It is demonstrated that H2S is an important endogenous vasoactive factor and the first identified gaseous opener of KATP channels in vascular SMCs and production from vascular tissues was enhanced by nitric oxide.
Journal ArticleDOI
Hydrogen sulphide and its therapeutic potential
TL;DR: The physiology and biochemistry of H2S is overviews, the effects of H 2S inhibitors or H2s donors in animal models of disease are summarized, the potential options for the therapeutic exploitation of H1S are outlined and they are outlined.
Journal ArticleDOI
Protein S-nitrosylation: a physiological signal for neuronal nitric oxide.
Samie R. Jaffrey,Hediye Erdjument-Bromage,Christopher D. Ferris,Paul Tempst,Solomon H. Snyder +4 more
TL;DR: Protein S-nitrosylation is established as a physiological signalling mechanism for neuronally generated NO in mice harbouring a genomic deletion of neuronal NO synthase (nNOS).
Related Papers (5)
The possible role of hydrogen sulfide as an endogenous neuromodulator
Kazuho Abe,Hideo Kimura +1 more