H2S Signals Through Protein S-Sulfhydration
Asif K. Mustafa,Moataz M. Gadalla,Nilkantha Sen,Seyun Kim,Weitong Mu,Sadia K. Gazi,Roxanne K. Barrow,Guangdong Yang,Rui Wang,Solomon H. Snyder +9 more
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TLDR
Ex vivo endogenous H2S physiologically modifies cysteine residues in many proteins, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin, converting Cysteine -SH groups to -SSH groups in a process the authors call S-sulfhydration.Abstract:
Hydrogen sulfide (H2S), a messenger molecule generated by cystathionine gamma-lyase, acts as a physiologic vasorelaxant. Mechanisms whereby H2S signals have been elusive. We now show that H2S physiologically modifies cysteines in a large number of proteins by S-sulfhydration. About 10 to 25% of many liver proteins, including actin, tubulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are sulfhydrated under physiological conditions. Sulfhydration augments GAPDH activity and enhances actin polymerization. Sulfhydration thus appears to be a physiologic posttranslational modification for proteins.read more
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Developing Polysulfide-Sensitive GFPs for Real-Time Analysis of Polysulfides in Live Cells and Subcellular Organelles.
Xin Hu,Huanjie Li,Xi Zhang,Zhigang Chen,Rui Zhao,Ningke Hou,Jihua Liu,Luying Xun,Luying Xun,Huaiwei Liu +9 more
TL;DR: These probes provided long-desired tools for polysulfide in vivo studies and displayed ratiometric changes to intracellularPolysulfides that had clear variations associated with the growth phases, suggesting that polys sulfurides have multiple origins and functions in cells.
Journal ArticleDOI
Sulfide affects the mitochondrial respiration, the Ca2+-activated F1FO-ATPase activity and the permeability transition pore but does not change the Mg2+-activated F1FO-ATPase activity in swine heart mitochondria.
Salvatore Nesci,Cristina Algieri,Fabiana Trombetti,Vittoria Ventrella,Micaela Fabbri,Alessandra Pagliarani +5 more
TL;DR: In this paper, the H2S donor NaHS, at micromolar concentrations, was tested on swine heart mitochondria, and the results showed that the NaHS-activated F1FO-ATPase activated by the natural cofactor Mg2+ showed a slight 15% enzyme inhibition at 100 µM NaHS.
Journal ArticleDOI
Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro.
Jan Nevoral,Tereza Žalmanová,K. Zámostná,Tomáš Kott,Veronika Kucerova-Chrpova,Jean-François Bodart,Armance Gelaude,Radek Prochazka,Matyáš Orsák,Miloslav Šulc,Pavel Klein,M. Dvořáková,Ivona Weingartova,Aurélia Víghová,Kristýna Hošková,Tereza Krejcova,František Jílek,Jaroslav Petr +17 more
TL;DR: Hydrogen sulfide appears to be an important cell messenger during mammalian oocyte meiosis and adequate cumulus expansion during oocyte maturation and adequate Cumulus expansion.
Journal ArticleDOI
Hydrogen sulfide: a target to modulate oxidative stress and neuroplasticity for the treatment of pathological anxiety.
TL;DR: The current challenge in H2S therapeutics remains finding an efficient delivery system of this gasotransmitter in a reliable, safe and nontoxic form to engage in clinical trials.
Journal ArticleDOI
Abnormalities of hydrogen sulfide and glutathione pathways in mitochondrial dysfunction.
TL;DR: A review of the recent evidences of derangement of the metabolism of H2S, at biosynthesis or oxidation levels, in mitochondrial dysfunction, focusing specifically on the alterations of H 2S oxidation caused by primary Coenzyme Q (CoQ) deficiency is presented in this paper.
References
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Journal ArticleDOI
H2S as a Physiologic Vasorelaxant: Hypertension in Mice with Deletion of Cystathionine γ-Lyase
Guangdong Yang,Guangdong Yang,Lingyun Wu,Bo Jiang,Wei Yang,Jiansong Qi,Kun Cao,Qinghe Meng,Asif K. Mustafa,Weitong Mu,Shengming Zhang,Solomon H. Snyder,Rui Wang,Rui Wang +13 more
TL;DR: It is shown that H2S is physiologically generated by cystathionine γ-lyase (CSE) and that genetic deletion of this enzyme in mice markedly reduces H 2S levels in the serum, heart, aorta, and other tissues.
Journal ArticleDOI
Protein S-nitrosylation: purview and parameters.
Douglas T. Hess,Akio Matsumoto,Sung Oog Kim,Harvey E. Marshall,Jonathan S. Stamler,Jonathan S. Stamler +5 more
TL;DR: S-nitrosylation conveys a large part of the ubiquitous influence of nitric oxide on cellular signal transduction, and provides a mechanism for redox-based physiological regulation.
Journal ArticleDOI
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
TL;DR: It is demonstrated that H2S is an important endogenous vasoactive factor and the first identified gaseous opener of KATP channels in vascular SMCs and production from vascular tissues was enhanced by nitric oxide.
Journal ArticleDOI
Hydrogen sulphide and its therapeutic potential
TL;DR: The physiology and biochemistry of H2S is overviews, the effects of H 2S inhibitors or H2s donors in animal models of disease are summarized, the potential options for the therapeutic exploitation of H1S are outlined and they are outlined.
Journal ArticleDOI
Protein S-nitrosylation: a physiological signal for neuronal nitric oxide.
Samie R. Jaffrey,Hediye Erdjument-Bromage,Christopher D. Ferris,Paul Tempst,Solomon H. Snyder +4 more
TL;DR: Protein S-nitrosylation is established as a physiological signalling mechanism for neuronally generated NO in mice harbouring a genomic deletion of neuronal NO synthase (nNOS).
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