Journal ArticleDOI
Highly sensitive immunoassay based on immunogold-silver amplification and inductively coupled plasma mass spectrometric detection.
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TLDR
A highly sensitive inductively coupled plasma mass spectrometric (ICPMS) method for the determination of human carcinoembryonic antigen (CEA) was demonstrated, which combined the inherent high sensitivity of elemental mass Spectrometric measurement with the signal amplification of catalytic silver deposition on immunogold tags.Abstract:
In this work, we demonstrated a highly sensitive inductively coupled plasma mass spectrometric (ICPMS) method for the determination of human carcinoembryonic antigen (CEA), which combined the inherent high sensitivity of elemental mass spectrometric measurement with the signal amplification of catalytic silver deposition on immunogold tags. The silver amplification procedure was easy to handle and required cheap reagents, and the sensitivity was greatly enhanced to 60-fold after a 15 min silver amplification procedure. The experimental conditions, including detection of gold and silver by ICPMS, immunoassay parameters, silver amplification parameters, analytical performance, and clinical serum samples analysis, were investigated. The ICPMS Ag signal intensity depends linearly on the logarithm of the concentration of human CEA over the range of 0.07−1000 ng mL−1 with a limit of detection (LOD, 3σ) of 0.03 ng mL−1 (i.e., 0.15 pM). The LOD of the proposed method is around 2 orders of magnitude lower than tha...read more
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The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing.
David H. Wilson,David M. Rissin,Cheuk W. Kan,David Fournier,Tomasz Piech,Todd G. Campbell,Raymond E. Meyer,Matthew W. Fishburn,Carlos Cabrera,Purvish P. Patel,Erica Frew,Yao Chen,Lei Chang,Evan P. Ferrell,Volker von Einem,William McGuigan,Marcus Reinhardt,Heiko Sayer,Claus Vielsack,David C. Duffy +19 more
TL;DR: A new laboratory instrument is described that provides full automation of single-molecule array (Simoa) technology for digital immunoassays that was illustrated in two clinical areas: traumatic brain injury and early detection of infectious disease.
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Functional nanoprobes for ultrasensitive detection of biomolecules: an update
TL;DR: This review provides an update on the recent advances in functional nanoprobes with biomolecules for highly sensitive and selective biosensing, particularly in the past two years, which reflects new progress since the publication of the last review on the same topic in Chem.
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Lateral-flow immunoassays for mycotoxins and phycotoxins: a review
TL;DR: This review focuses on new aspects of the development and optimization of lateral-flow devices for mycotoxin and phycotoxin detection, including strategies for management of matrix interference and for investigation of the improvements achieved by signal-enhancing strategies or by application of non-gold nanoparticle signal reporters.
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A novel label-free electrochemical immunosensor based on functionalized nitrogen-doped graphene quantum dots for carcinoembryonic antigen detection.
Yuying Yang,Qing Liu,Yan Liu,Jianjian Cui,Hui Liu,Ping Wang,Yueyun Li,Lei Chen,Zengdian Zhao,Yunhui Dong +9 more
TL;DR: A novel and ultrasensitive label-free electrochemical immunosensor possesses high sensitivity, special selectivity and long-term stability, which shows promising application in bioassay analysis.
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Lateral flow based immunobiosensors for detection of food contaminants.
Mohammadjavad Raeisossadati,Noor Mohammad Danesh,Fazlollah Borna,Mehrdad Gholamzad,Mohammad Ramezani,Khalil Abnous,Seyed Mohammad Taghdisi +6 more
TL;DR: This review article surveyed application of lateral flow biosensors in detection of different food contaminants and labels used to enhance the efficiency of the system.
References
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Journal ArticleDOI
Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection
TL;DR: Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms, and the current unoptimized detection limit of this method is 20 femtomolar.
Journal ArticleDOI
Scanometric DNA Array Detection with Nanoparticle Probes
TL;DR: When coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.
Journal ArticleDOI
Direct observation of individual endogenous protein complexes in situ by proximity ligation
Ola Söderberg,Mats Gullberg,Malin Jarvius,Karin Ridderstråle,Karl-Johan Leuchowius,Jonas Jarvius,Kenneth Wester,Per Hydbring,Fuad Bahram,Lars-Gunnar Larsson,Ulf Landegren +10 more
TL;DR: This method is used to show specific regulation of protein-protein interactions between endogenous Myc and Max oncogenic transcription factors in response to interferon-γ (IFN-γ) signaling and low-molecular-weight inhibitors.
Journal ArticleDOI
Biomarkers in cancer staging, prognosis and treatment selection.
TL;DR: Advances in genomics, proteomics and molecular pathology have generated many candidate biomarkers with potential clinical value, but how and when they can be integrated into clinical care is crucial.
Journal ArticleDOI
Array-based electrical detection of DNA with nanoparticle probes
TL;DR: Using this method, target DNA is detected at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1 and an unusual salt concentration–dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash.