Journal ArticleDOI
Highly Sensitive MicroRNA Detection by Coupling Nicking-Enhanced Rolling Circle Amplification with MoS2 Quantum Dots.
Jia Ge,Yun Hu,Ruijie Deng,Zhaohui Li,Kaixiang Zhang,Muling Shi,Dan Yang,Ren Cai,Weihong Tan,Weihong Tan +9 more
TLDR
The as-prepared sensor was applied for detecting microRNA let-7a in dilute human serum samples and achieved a satisfactory recovery rate, demonstrating its potential in clinic diagnosis of microRNA-associated disease and biochemical research.Abstract:
In this work, a label-free and highly sensitive fluorescence assay was constructed for microRNA detection. Nicking-enhanced rolling circle amplification (RCA) induced by G-quadruplex formation is coupled with inner filter effect (IFE)-based quenching effects of MoS2 quantum dots (MoS2 QDs). The padlock probe contains a recognition sequence to target microRNA and an accessible nicking site. The padlock probe is cyclized upon hybridization with target microRNA. Sequentially, amplification initiates a production of a long-concatenated sequence of circular probes. Abundant G-quadruplex sequences are produced via the nicking process and then used as the trigger to initiate the next RCA. In the presence of hemin, numerous hemin/G-quadruplex DNAzymes are formed, which catalyze the oxidation of o-phenylenediamine (OPD) into the colored product 2,3-diaminophenazine, resulting in quenching of the fluorescence of MoS2 QDs. This sensing strategy enables detection of microRNA let-7a with high selectivity and a detection limit of 4.6 fM. The as-prepared sensor was applied for detecting microRNA let-7a in dilute human serum samples and achieved a satisfactory recovery rate, demonstrating its potential in clinic diagnosis of microRNA-associated disease and biochemical research.read more
Citations
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Journal ArticleDOI
Rolling Circle Replication for Biosensing, Bioimaging, and Biomedicine
TL;DR: This review introduces the fundamentals of RCR and describes the most recent advances in RCR-based detection methods and delivery vehicles for biosensing, bioimaging, and biomedicine.
Journal ArticleDOI
Heterojunction of Vertically Arrayed MoS2 Nanosheet/N-Doped Reduced Graphene Oxide Enabling a Nanozyme for Sensitive Biomolecule Monitoring.
Longwei Wang,Longwei Wang,Bo Li,Zhen You,Aizhu Wang,Xuanyu Chen,Gaojing Song,Ling Yang,Dan Chen,Xin Yu,Jing Liu,Jing Liu,Chunying Chen +12 more
TL;DR: In this paper, a peroxidase-like heterostructured nanozyme, vertically arraying molybdenum disulfide nanosheets on a substrate layer of nitrogen-doped reduced graphene oxide (MoS2/N-rGO), with a well-pleasing stability that is characterized by the retained enzymatic activity and maintained structure after 2 years of casual storage at ambient temperatures or 80 cycles of catalytic reaction.
Journal ArticleDOI
N-doped carbon dots for highly sensitive and selective sensing of copper ion and sulfide anion in lake water
TL;DR: A facile fluorescent bioassay for copper ions (Cu2+) and sulfide anions (S2-) was developed in this article based on fluorescent nitrogen-doped carbon dots (N-CDs).
Journal ArticleDOI
Recent advances in catalytic hairpin assembly signal amplification-based sensing strategies for microRNA detection.
TL;DR: In this article, the authors describe the characteristics and mechanism of CHA signal amplification and classify the CHA-based miRNA sensing strategies into several categories based on the "signal conversion substance", including fluorophores, enzymes, nanomaterials, and nucleotide sequences.
Journal ArticleDOI
Programmable DNA Circuits for Flexible and Robust Exciton-Plasmon Interaction-Based Photoelectrochemical Biosensing.
Hongbo Li,Ye Cao,Tianyu Wu,Yansong Zhang,Zhaoting Zheng,Jingchun Lv,Airong Mao,Yuye Zhang,Qin Tang,Jing Li +9 more
TL;DR: A novel and elegant exciton-plasmon interaction (EPI)-based photoelectrochemical (PEC) biosensor was developed with the assistance of a programmable entropy-driven DNA amplifier and superparamagnetic nanostructures, which grants the PEC biosensor with ultrasensitivity and high selectivity.
References
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Emerging Photoluminescence in Monolayer MoS2
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TL;DR: This observation shows that quantum confinement in layered d-electron materials like MoS(2), a prototypical metal dichalcogenide, provides new opportunities for engineering the electronic structure of matter at the nanoscale.
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MicroRNAs: small RNAs with a big role in gene regulation
Lin He,Gregory J. Hannon +1 more
TL;DR: Two founding members of the microRNA family were originally identified in Caenorhabditis elegans as genes that were required for the timed regulation of developmental events and indicate the existence of multiple RISCs that carry out related but specific biological functions.
Journal ArticleDOI
Photoluminescence from Chemically Exfoliated MoS2
Goki Eda,Goki Eda,Hisato Yamaguchi,Damien Voiry,Takeshi Fujita,Mingwei Chen,Manishkumar Chhowalla +6 more
TL;DR: Above an annealing temperature of 300 °C, chemically exfoliated MoS2 exhibit prominent band gap photoluminescence, similar to mechanically exfoliate monolayers, indicating that their semiconducting properties are largely restored.
Journal ArticleDOI
Therapeutic targeting of microRNAs: current status and future challenges
Zhonghan Li,Tariq M. Rana +1 more
TL;DR: Current knowledge of the design and performance of chemically modified miRNA-targeting antisense oligonucleotides is summarized, various in vivo delivery strategies are discussed and ongoing challenges to ensure the specificity and efficacy of therapeutic oligon nucleotides in vivo are analysed.