Journal ArticleDOI
Human p53 gene localized to short arm of chromosome 17
Carl W. Miller,T. K. Mohandas,David E. Wolf,Miron Prokocimer,Varda Rotter,H. Phillip Koeffler +5 more
TLDR
Ch Chromosomal localization may provide a better understanding of the relationship of p53 to other human cellular genes and of its possible role in malignancies associated with specific chromosomal rearrangements.Abstract:
The p53 gene codes for a nuclear protein that has an important role in normal cellular replication. The concentration of p53 protein is frequently elevated in transformed cells. Transfection studies show that the p53 gene, in collaboration with the activated ras oncogene, can transform cells. Chromosomal localization may provide a better understanding of the relationship of p53 to other human cellular genes and of its possible role in malignancies associated with specific chromosomal rearrangements. A recent study mapped the human p53 gene to the long arm of chromosome 17 (17q21-q22) using in situ chromosomal hybridization. Here, by Southern filter hybridization of DNAs from human-rodent hybrids, we have localized the p53 gene to the short arm of human chromosome 17.read more
Citations
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The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression
TL;DR: The HL-60 cell line, derived from a single patient with acute promyelocytic leukemia, provides a unique in vitro model system for studying the cellular and molecular events involved in the proliferation and differentiation of normal and leukemic cells of the granulocyte/monocyte/macrophage lineage.
Journal ArticleDOI
Mutations in the p53 Tumor Suppressor Gene: Important Milestones at the Various Steps of Tumorigenesis
TL;DR: Interestingly, mutations in the p53 gene were shown to occur at different phases of the multistep process of malignant transformation, thus contributing differentially to tumor initiation, promotion, aggressiveness, and metastasis.
Journal ArticleDOI
Twenty years of p53 research: structural and functional aspects of the p53 protein.
Pierre May,Evelyne May +1 more
TL;DR: The aim of this review is to relate how the p53 history has unfolded until now, and to underscore the present knowledge of this paradigmatic protein.
Journal ArticleDOI
Rearrangement of the p53 gene in human osteogenic sarcomas.
TL;DR: A survey of 134 human carcinomas, sarcomas, leukemias, and lymphomas obtained at surgery or from peripheral blood found rearrangements of the p53 gene only in osteogenic sarcoms, indicating that the genetic abnormality in the tumor was acquired.
Journal ArticleDOI
Amplification of the neu (c-erbB-2) oncogene in human mammmary tumors is relatively frequent and is often accompanied by amplification of the linked c-erbA oncogene.
M.J. van de Vijver,R van de Bersselaar,Peter Devilee,Cees J. Cornelisse,Johannes L. Peterse,Roel Nusse +5 more
TL;DR: The relatively high frequency of neu amplification points to a functional role in human breast cancer and coamplification of the c-erbA oncogene could contribute to this disease as well but is most likely fortuitous.
References
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Journal ArticleDOI
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
TL;DR: Ten percent dextran sulfate accelerates the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100-fold, without increasing the background significantly.
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Participation of p53 cellular tumour antigen in transformation of normal embryonic cells.
TL;DR: It is demonstrated here that p53 can cooperate with the activated Ha-ras oncogene to transform normal embryonic cells, and the resultant foci contain cells of a markedly altered morphology which produce high levels of p53.
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Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation
TL;DR: The results indicate that the p53-encoding gene can play a causal role in the conversion of normal fibroblasts into tumorigenic cells, suggesting an important role of p53 in tumorigenesis.
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Cellular immortalization by a cDNA clone encoding the transformation-associated phosphoprotein p53.
TL;DR: Cloned complementary DNA sequences encoding murine p5318 are cloned and reported here that transfection of p53 expression constructs into cells of finite lifespan in vitro results in cellular immortality and susceptibility to transformation by a ras oncogene.
Journal ArticleDOI
Growth regulation of a cellular tumour antigen, p53, in nontransformed cells.
Nancy C. Reich,Arnold J. Levine +1 more
TL;DR: An increase in the synthesis and steady-state levels of p53 protein and mRNA prior to DNA synthesis in late G1 is demonstrated and a role for p53 in the progression of cells from a growth-arrested state to an actively dividing state is suggested.