Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum
Sérgio Simões,Sérgio Simões,Vladimir Slepushkin,Pedro Pires,Rogério Gaspar,Maria C. Pedroso de Lima,Nejat Düzgüneş +6 more
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TLDR
The results indicate that negatively charged HSA-lipoplexes can facilitate efficient transfection of cultured cells, and that they may overcome some of the problems associated with the use of highly positively charged complexes for gene delivery in vivo.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2000-02-15 and is currently open access. It has received 141 citations till now. The article focuses on the topics: Cationic liposome & Gene delivery.read more
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Albumin-based nanoparticles as potential controlled release drug delivery systems.
TL;DR: The current review embodies an in-depth discussion of albumin nanoparticles with respect to types, formulation aspects, major outcomes of in vitro and in vivo investigations as well as site-specific drug targeting using various ligands modifying the surface of albumins with special insights to the field of oncology.
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Progress in developing cationic vectors for non-viral systemic gene therapy against cancer.
TL;DR: The aim of this review is to describe the "perfect vector" for systemic gene therapy against cancer based on the advantages and disadvantages of existing vectors and on the hurdles encountered with these carriers.
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Gene delivery by lipoplexes and polyplexes
TL;DR: The interactions between the vectors and DNA leading to complex formation, the supramolecular structures of lipoplexes and polyplexes, and their mechanisms of DNA transfer are reviewed to provide a framework for the future design and synthesis of optimal non-viral vectors for gene therapy.
Journal ArticleDOI
Cationic lipids, lipoplexes and intracellular delivery of genes
Luc Wasungu,Dick Hoekstra +1 more
TL;DR: Some recent developments in the field on the structure/function relationship of cationic lipids in the mechanism of transfection and a comparison with mechanisms of viral and polyplex-mediated gene delivery are discussed.
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Water Soluble Polymers for Pharmaceutical Applications
TL;DR: The general properties and applications of different water soluble polymers in the formulation of different dosage forms, novel delivery systems and biomedical applications will be discussed.
References
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Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
TL;DR: The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.
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T Lymphocyte-Directed Gene Therapy for ADA− SCID: Initial Trial Results After 4 Years
R. Michael Blaese,Kenneth W. Culver,A. Dusty Miller,Charles S. Carter,Thomas A. Fleisher,Mario Clerici,Gene M. Shearer,Lauren A. Chang,Yawen Chiang,Paul Tolstoshev,Jay J. Greenblatt,Steven A. Rosenberg,Harvey G. Klein,Melvin Berger,Craig A. Mullen,W. Jay Ramsey,Linda M. Muul,Richard A. Morgan,W. French Anderson +18 more
TL;DR: In this article, a clinical trial was started using retroviral-mediated transfer of the ADA gene into the T cells of two children with severe combined immunodeficiency (ADA-SCID).
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Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.
Yuhong Xu,Francis C. Szoka +1 more
TL;DR: It is proposed that after the cationic lipid/DNA complex is internalized into cells by endocytosis it destabilization induces flip-flop of anionic lipids from the cytoplasmic-facing monolayer, which laterally diffuse into the complex and form a charge neutral ion pair with the cationsic lipids.
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Systemic gene expression after intravenous DNA delivery into adult mice
TL;DR: A single intravenous injection of expression plasmid:cationic liposome complexes into adult mice efficiently transfected virtually all tissues and most of the extravascular parenchymal cells expressed the transgene without any apparent treatment-related toxicity.
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Long-term restoration of immunity against Epstein-Barr virus infection by adoptive transfer of gene-modified virus-specific T lymphocytes
Helen E. Heslop,Catherine Y.C. Ng,Congfen Li,Colton Smith,Susan K. Loftin,Robert A. Krance,Robert A. Krance,Malcolm K. Brenner,Malcolm K. Brenner,Cliona M. Rooney,Cliona M. Rooney +10 more
TL;DR: These findings support wider use of antigen–specific CTLs in adoptive immunotherapy and restore cellular immune responses against EBV, but also established populations of CTL precursors that could respond to in vivo or ex vivo challenge with the virus for as long as 18 months.