Journal ArticleDOI
Gene delivery by lipoplexes and polyplexes
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TLDR
The interactions between the vectors and DNA leading to complex formation, the supramolecular structures of lipoplexes and polyplexes, and their mechanisms of DNA transfer are reviewed to provide a framework for the future design and synthesis of optimal non-viral vectors for gene therapy.About:
This article is published in European Journal of Pharmaceutical Sciences.The article was published on 2010-06-14. It has received 594 citations till now. The article focuses on the topics: Gene delivery & Vectors in gene therapy.read more
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Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
Journal ArticleDOI
Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: Structure, Preparation and Application.
TL;DR: A wide discussion about preparation methods, advantages, disadvantages and applications of LNPs is presented by focusing on SLNs and NLCs, two major types of Lipid-based nanoparticles.
Journal ArticleDOI
Lipid–polymer hybrid nanoparticles as a new generation therapeutic delivery platform: A review
TL;DR: The current state of development for the LPNs preparation and applications is detailed, from which future research works needed to bring theLPNs closer to its clinical realization are identified.
Journal ArticleDOI
Chemistry, Structures, and Advanced Applications of Nanocomposites from Biorenewable Resources.
Burhan Ates,Süleyman Köytepe,Ahmet Ulu,Canbolat Gürses,Vijay Kumar Thakur,Vijay Kumar Thakur,Vijay Kumar Thakur +6 more
TL;DR: This comprehensive review provides chemistry, structures, advanced applications, and recent developments about nanocomposites obtained from biorenewable sources.
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Cationic Antimicrobial Polymers and Their Assemblies
TL;DR: Antimicrobial polymers grafted or self-assembled to inert or non inert vehicles can yield hybrid antimicrobial nanostructures or films, which can act as antimicrobials by themselves or deliver bioactive molecules for a variety of applications.
References
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Journal ArticleDOI
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
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Diffusion of Univalent Ions Across the Lamellae of Swollen Phospholipids
TL;DR: It is found that as the surface charge of the lipid lamellae is increased, the amount of cation per μmle of lipid increases, and the phospholipid liquid crystalline structures appear to “bind” or “capture” cations.
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Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation
TL;DR: A substantial fraction of the aqueous phase is entrapped within the vesicles, encapsulating even large macromolecular assemblies with high efficiency, and has unique advantages for encapsulating valuable water-soluble materials such as drugs, proteins, nucleic acids, and other biochemical reagents.
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Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
TL;DR: The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.