![Figure 2 : Structure of current cationic lipids used in gene therapy and the helper lipid DOPE. DOTMA: N [1-(2,3-dioleyloxy) propyl]-N,N,N-trymethylamonium chloride, DOTAP: 1,2-dioleyl-3-trimethylamonium-propane, DMRIE: N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy-1-propananium bromide), DOTIM : 1-[2-(oleyloxy)ethyl]-2-oleyl-3-(2-hydroxyethyl)imidazolinium chloride DOGS: dioctadecylamidoglycylspermine DC-Chol: [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol BGTC: bis-guanidium-tren-cholesterol DOPE: 1,2-dyoleyl-sn-glycerol-3-phosphoethanolamine](/figures/figure-2-structure-of-current-cationic-lipids-used-in-gene-2yq9ng5a.webp)
![Table 2 : Example of promising systems for systemic gene delivery. mAb: monoclonal antibody, scFv: single-chain antibody fragment, pDNA : plasmidic DNA, asODN: antisens oligodeoxynucleotides, siRNA: small interfering RNA, DSPE-PEG: 1,2-distearoylglycero-3- phosphatidylethanolamine-N- polyethylene glycol, PEG-Cer : PEG-ceramide, PEG-DAG: PEG-Diacylglycerol, PEG-C-DMA: 3-N[(methoxypoly(ethyleneglycol) 2000) carbamoyl]-1,2-dimyristyloxy-propylamine, MAL-PEG-NHS: Maleimide-PEG-hydroxysuccinimide, AtuFECT01 : cationic lipid, DPhyPE: 1,2- diphytanoyl-sn-glycero-3-phosphoethanolamine, DTNS : DNA nuclear targeting signal, NLS: nuclear localization signal peptide. SV40 nuclear localization signal peptide](/figures/table-2-example-of-promising-systems-for-systemic-gene-1ehsl5ku.webp)
Q2. What is the name of the author?
The understanding of the molecular mechanisms involved in cancer and the development of nucleic acid delivery systems are two concepts that have led to this development.
Progress in developing cationic vectors for non-viral systemic gene therapy against cancer.
TLDR
The aim of this review is to describe the "perfect vector" for systemic gene therapy against cancer based on the advantages and disadvantages of existing vectors and on the hurdles encountered with these carriers.About:
This article is published in Biomaterials.The article was published on 2008-08-01 and is currently open access. It has received 787 citations till now. The article focuses on the topics: Vector (molecular biology) & Gene delivery.read more
Figures
![Figure 2 : Structure of current cationic lipids used in gene therapy and the helper lipid DOPE. DOTMA: N [1-(2,3-dioleyloxy) propyl]-N,N,N-trymethylamonium chloride, DOTAP: 1,2-dioleyl-3-trimethylamonium-propane, DMRIE: N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy-1-propananium bromide), DOTIM : 1-[2-(oleyloxy)ethyl]-2-oleyl-3-(2-hydroxyethyl)imidazolinium chloride DOGS: dioctadecylamidoglycylspermine DC-Chol: [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol BGTC: bis-guanidium-tren-cholesterol DOPE: 1,2-dyoleyl-sn-glycerol-3-phosphoethanolamine](/figures/figure-2-structure-of-current-cationic-lipids-used-in-gene-2yq9ng5a.png)
Figure 2 : Structure of current cationic lipids used in gene therapy and the helper lipid DOPE. DOTMA: N [1-(2,3-dioleyloxy) propyl]-N,N,N-trymethylamonium chloride, DOTAP: 1,2-dioleyl-3-trimethylamonium-propane, DMRIE: N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy-1-propananium bromide), DOTIM : 1-[2-(oleyloxy)ethyl]-2-oleyl-3-(2-hydroxyethyl)imidazolinium chloride DOGS: dioctadecylamidoglycylspermine DC-Chol: [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol BGTC: bis-guanidium-tren-cholesterol DOPE: 1,2-dyoleyl-sn-glycerol-3-phosphoethanolamine ![Table 2 : Example of promising systems for systemic gene delivery. mAb: monoclonal antibody, scFv: single-chain antibody fragment, pDNA : plasmidic DNA, asODN: antisens oligodeoxynucleotides, siRNA: small interfering RNA, DSPE-PEG: 1,2-distearoylglycero-3- phosphatidylethanolamine-N- polyethylene glycol, PEG-Cer : PEG-ceramide, PEG-DAG: PEG-Diacylglycerol, PEG-C-DMA: 3-N[(methoxypoly(ethyleneglycol) 2000) carbamoyl]-1,2-dimyristyloxy-propylamine, MAL-PEG-NHS: Maleimide-PEG-hydroxysuccinimide, AtuFECT01 : cationic lipid, DPhyPE: 1,2- diphytanoyl-sn-glycero-3-phosphoethanolamine, DTNS : DNA nuclear targeting signal, NLS: nuclear localization signal peptide. SV40 nuclear localization signal peptide](/figures/table-2-example-of-promising-systems-for-systemic-gene-1ehsl5ku.png)
Table 2 : Example of promising systems for systemic gene delivery. mAb: monoclonal antibody, scFv: single-chain antibody fragment, pDNA : plasmidic DNA, asODN: antisens oligodeoxynucleotides, siRNA: small interfering RNA, DSPE-PEG: 1,2-distearoylglycero-3- phosphatidylethanolamine-N- polyethylene glycol, PEG-Cer : PEG-ceramide, PEG-DAG: PEG-Diacylglycerol, PEG-C-DMA: 3-N[(methoxypoly(ethyleneglycol) 2000) carbamoyl]-1,2-dimyristyloxy-propylamine, MAL-PEG-NHS: Maleimide-PEG-hydroxysuccinimide, AtuFECT01 : cationic lipid, DPhyPE: 1,2- diphytanoyl-sn-glycero-3-phosphoethanolamine, DTNS : DNA nuclear targeting signal, NLS: nuclear localization signal peptide. SV40 nuclear localization signal peptide 
Figure 1 : Structures of current cationic polymers used in gene therapy. PEI = poly(ethyleneimine) PLL = poly(L-Lysine) PAMAM = poly(amidoamine) 
Figure 5: Hypothesis of endosomal escape of lipoplexes and polyplexes gene delivery systems. 
Figure 4: Schematic representation of the different hurdles encountered by a gene delivery system to enter and traffic into a tumor cell. 
Figure 6 : Schematic nuclear entry mechanism through Nuclear Pore Complexes (NPC).
Citations
More filters
Journal ArticleDOI
Non-viral vectors for gene-based therapy
Hao Yin,Rosemary Lynn Kanasty,Ahmed A. Eltoukhy,Arturo J. Vegas,J. Robert Dorkin,Daniel G. Anderson +5 more
TL;DR: The biological barriers to gene delivery in vivo are introduced and recent advances in material sciences, nanotechnology and nucleic acid chemistry that have yielded promising non-viral delivery systems are discussed, some of which are currently undergoing testing in clinical trials.
Journal ArticleDOI
Nanochemistry and Nanomedicine for Nanoparticle-based Diagnostics and Therapy
TL;DR: This work presents a new generation of high-performance liquid chromatography platforms for selective separation of Na6(CO3) from Na4(SO4) through Na2SO4 and shows real-world applications in drug discovery and treatment of central nervous system disorders.
Journal ArticleDOI
Iron Oxide Based Nanoparticles for Multimodal Imaging and Magnetoresponsive Therapy.
TL;DR: Magnetoresponsive Therapy Nohyun Lee, Dongwon Yoo, Daishun Ling,†,‡,⊥ Mi Hyeon Cho, Taeghwan H Yeon,*,†,† and Jinwoo Cheon.
Journal ArticleDOI
Understanding the role of surface charges in cellular adsorption versus internalization by selectively removing gold nanoparticles on the cell surface with a I2/KI etchant.
TL;DR: An etching solution based on I2 and KI that can selectively dissolve the Au nanospheres on the cell surface within a short period of time is introduced that is capable of etching away a relatively large amount of Au Nanospheres at a low molar concentration.
Journal Article
Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
Joanna Rejman,Massimo Conese +1 more
TL;DR: In this article, the authors investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and K(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of FITC-poly-l-lysine-labeled DOTAP/DNA lipoplexes and PEI/DNA polyplexes by A549 pneumocytes and HeLa cells and on the transfection efficiencies of these complexes with the luciferase gene.
References
More filters
Journal ArticleDOI
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
Journal ArticleDOI
Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
TL;DR: It is shown that mammalian TLR3 recognizes dsRNA, and that activation of the receptor induces the activation of NF-κB and the production of type I interferons (IFNs).
Journal ArticleDOI
Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review.
TL;DR: The basic characteristics of the EPR effect, particularly the factors involved, are described, as well as its modulation for improving delivery of macromolecular drugs to the tumor.
Journal ArticleDOI
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
Journal ArticleDOI
Species-Specific Recognition of Single-Stranded RNA via Toll-like Receptor 7 and 8
Florian Heil,Hiroaki Hemmi,Hubertus Hochrein,Franziska Ampenberger,Carsten J. Kirschning,Shizuo Akira,Grayson B. Lipford,Hermann Wagner,Stefan Bauer +8 more
TL;DR: It is shown that guanosine (G)- and uridine (U)-rich ssRNA oligonucleotides derived from human immunodeficiency virus–1 (HIV-1) stimulate dendritic cells and macrophages to secrete interferon-α and proinflammatory, as well as regulatory, cytokines, and these data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8.