Journal ArticleDOI
Hydrolysis of Cellulose Using Mono-Component Enzymes Shows Synergy during Hydrolysis of Phosphoric Acid Swollen Cellulose (PASC), but Competition on Avicel
Natalija Andersen,Katja Salomon Johansen,Michael Locht Michelsen,Erling Halfdan Stenby,Kristian B. R. M. Krogh,Lisbeth Olsson +5 more
TLDR
In this paper, the synergy between three groups of cellulolytic enzymes, 20 mixtures of different mole percentage of Humicola insolens Cel45A (EG V) and Cel6A (CBH II), and β-glucosidase was used to hydrolyze Avicel and phosphoric acid swollen cellulose/Avicel (PASC).About:
This article is published in Enzyme and Microbial Technology.The article was published on 2008-03-04. It has received 101 citations till now. The article focuses on the topics: Cellulase & Hydrolysis.read more
Citations
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Journal ArticleDOI
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
TL;DR: Four different techniques incorporating X-ray diffraction and solid-state 13C nuclear magnetic resonance (NMR) were compared using eight different cellulose preparations and it was found that the simplest method, which is also the most widely used, and which involves measurement of just two heights in the X- Ray diffractogram, produced significantly higher crystallinity values than did the other methods.
Journal ArticleDOI
A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes--factors affecting enzymes, conversion and synergy.
J.S. Van Dyk,Brett I. Pletschke +1 more
TL;DR: This review examines the enzymes required to degrade various components of lignocellulose and the impact of pretreatments on the lignosic substrates and the enzyme required for degradation and the effect of and interaction between different hemicellulases on complex substrates.
Journal ArticleDOI
Cellulose crystallinity--a key predictor of the enzymatic hydrolysis rate
TL;DR: It is shown that the crystallinity of pure cellulosic Avicel stays constant during enzymatic conversion, which supports the determinant role of crystallinity rather than adsorption on the enzymatics rate.
Journal ArticleDOI
Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives.
TL;DR: Major pretreatment technologies and different enzyme process setups are reviewed and an in-depth discussion of the various enzyme types that are currently in use are presented, with ample attention to the role of the recently discovered lytic polysaccharide monooxygenases.
Journal ArticleDOI
The Contribution of Non-catalytic Carbohydrate Binding Modules to the Activity of Lytic Polysaccharide Monooxygenases
TL;DR: It is demonstrated that engineered LPMO-CBM hybrids can display enhanced industrially relevant oxygenations and can modulate the mode of action of LPMOs.
References
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Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
Microbial cellulose utilization: fundamentals and biotechnology.
TL;DR: A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts.
Journal ArticleDOI
Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems.
TL;DR: It is suggested that it is timely to revisit and reinvigorate functional modeling of cellulose hydrolysis and that this would be highly beneficial if not necessary in order to bring to bear the large volume of information available on cellulase components on the primary applications that motivate interest in the subject.
Journal ArticleDOI
Outlook for cellulase improvement: screening and selection strategies.
TL;DR: In this paper, the authors review quantitative cellulase activity assays using soluble and insoluble substrates, and focus on their advantages and limitations, and hypothesize that continuous culture using insoluble cellulosic substrates could be a powerful selection tool for enriching beneficial cellulase mutants from the large library displayed on the cell surface.
Related Papers (5)
Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems.
A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes--factors affecting enzymes, conversion and synergy.
J.S. Van Dyk,Brett I. Pletschke +1 more