Innovation in detection of microparticles and exosomes
TLDR
This review focuses on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small‐angle X‐ray scattering (SAXS), and anomalous SAXS.About:
This article is published in Journal of Thrombosis and Haemostasis.The article was published on 2013-06-01 and is currently open access. It has received 225 citations till now. The article focuses on the topics: Nanoparticle tracking analysis.read more
Citations
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Ectosomes and exosomes: shedding the confusion between extracellular vesicles
TL;DR: The similarities and differences between these two classes of vesicle are reviewed, suggesting that, despite their considerable differences, the functions of ectosomes may be largely analogous to those of exosomes.
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Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing.
E. van der Pol,Frank A. W. Coumans,Anita E. Grootemaat,Chris Gardiner,I.L. Sargent,Paul Harrison,A. Sturk,T. G. van Leeuwen,Rienk Nieuwland +8 more
TL;DR: Enumeration of extracellular vesicles has clinical potential as a biomarker for disease and currently employed techniques detect concentrations ranging from 104 to 1012 vesicle mL–1.
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Exosomes and Cardiac Repair After Myocardial Infarction
Susmita Sahoo,Douglas W. Losordo +1 more
TL;DR: This review critically examines the emerging role of exosomes in local and distant microcommunication mechanisms after myocardial infarction to bridge a major gap in knowledge of the repair mechanism afterMyocardial injury.
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Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine
TL;DR: A promising solution, microfluidic-based technologies that have incorporated a host of separation and sensing capabilities for exosome isolation, detection, and analysis, with emphasis on point-of-care and clinical applications are reviewed.
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Extracellular vesicles in coronary artery disease
Chantal M. Boulanger,Chantal M. Boulanger,Xavier Loyer,Xavier Loyer,Pierre-Emmanuel Rautou,Pierre-Emmanuel Rautou,Nicolas Amabile +6 more
TL;DR: Current knowledge on the role of extracellular vesicles in coronary artery disease, and their emerging potential as biomarkers and therapeutic agents are summarized.
References
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Book
Absorption and Scattering of Light by Small Particles
TL;DR: In this paper, a Potpourri of Particles is used to describe surface modes in small Particles and the Angular Dependence of Scattering is shown to be a function of the size of the particles.
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Classification, Functions, and Clinical Relevance of Extracellular Vesicles
TL;DR: Evidence is accumulating that vesicles are cargo containers used by eukaryotic cells to exchange biomolecules as transmembrane receptors and genetic information, and they have a myriad of potential clinical applications, ranging from biomarkers to anticancer therapy.
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Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
R Dragovic,Chris Gardiner,Alexandra S. Brooks,Dionne Tannetta,David J. P. Ferguson,Patrick Hole,Bob Carr,Christopher W.G. Redman,Adrian L. Harris,Peter J. Dobson,Paul Harrison,Ian L. Sargent +11 more
TL;DR: By combining NTA with fluorescence measurement it is demonstrated that vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined, demonstrating that NTA is far more sensitive than conventional flow cytometry.
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Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production
Eric Boilard,Peter A. Nigrovic,Peter A. Nigrovic,Katherine Larabee,Gerald F. Watts,Jonathan S. Coblyn,Michael E. Weinblatt,Elena Massarotti,Eileen Remold-O'Donnell,Richard W. Farndale,Jerry Ware,David M. Lee +11 more
TL;DR: A previously unappreciated role for platelets and their activation-induced microparticles in inflammatory joint diseases is demonstrated and the collagen receptor glycoprotein VI is identified as a key trigger for platelet microparticle generation in arthritis pathophysiology.