Integrated confocal and scanning probe microscopy for biomedical research.
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TLDR
A new hybrid AFM is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy and fluorescent imaging, to provide a more comprehensive understanding of biological systems.Abstract:
Atomic force microscopy (AFM) continues to be developed, not only in design, but also in application. The new focus of using AFM is changing from pure material to biomedical studies. More frequently, it is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy (CLSM) and fluorescent imaging, to provide a more comprehensive understanding of biological systems. To date, AFM has been used increasingly as a precise micromanipulator, probing and altering the mechanobiological characteristics of living cells and tissues, in order to examine specific, receptor-ligand interactions, material properties, and cell behavior. In this review, we discuss the development of this new hybrid AFM, current research, and potential applications in diagnosis and the detection of disease.read more
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Biomechanics and biophysics of cancer cells.
TL;DR: It is reasoned through experimental evidence that greater understanding of the mechanics of cancer cell deformability and its interactions with the extracellular physical, chemical and biological environments offers enormous potential for significant new developments in disease diagnostics, prophylactics, therapeutics and drug efficacy assays.
Journal ArticleDOI
Biomechanics and biophysics of cancer cells
TL;DR: It is reasoned through experimental evidence that greater understanding of the mechanics of cancer cell deformability and its interactions with the extracellular physical, chemical and biological environments offers enormous potential for significant new developments in disease diagnostics, prophylactics, therapeutics and drug efficacy assays.
Journal ArticleDOI
Compliance profile of the human cornea as measured by atomic force microscopy.
TL;DR: The results indicate that the biophysical properties, including elastic modulus, of each layer of the human cornea are unique and may play a role in the maintenance of homeostasis as well as in the response to therapeutic agents and disease states.
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Principles of Plant-Microbe Interactions
TL;DR: This book discusses pathogens and pest organisms which are a threat for plant growth, and highlights the roles which microbes can play in making agriculture and horticulture more sustainable.
Journal ArticleDOI
Atomic force microscopy comes of age
TL;DR: The current status of AFM in the field is reviewed and the important contribution AFM is poised to make in the understanding of biological systems is discussed.
References
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Atomic force microscope
TL;DR: The atomic force microscope as mentioned in this paper is a combination of the principles of the scanning tunneling microscope and the stylus profilometer, which was proposed as a method to measure forces as small as 10-18 N. As one application for this concept, they introduce a new type of microscope capable of investigating surfaces of insulators on an atomic scale.
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Detection and localization of individual antibody-antigen recognition events by atomic force microscopy
TL;DR: It is indicated that this methodology has promise for characterizing rate constants and kinetics of molecular recognition complexes and for molecular mapping of biosurfaces such as membranes.
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Drug-Induced Changes of Cytoskeletal Structure and Mechanics in Fibroblasts: An Atomic Force Microscopy Study
TL;DR: The effect of various drugs affecting the integrity of different components of the cytoskeleton on the elasticity of two fibroblast cell lines was investigated by elasticity measurements with an atomic force microscope (AFM).