Journal ArticleDOI
Interaction between quinolones antibiotics and bacterial outer membrane porin OmpF.
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TLDR
It is possible to conclude that some of the quinolones associate preferably with the protein than with simpler biomembrane models (liposomes), which suggests that an interaction drug/porin is, probably, the preferentially used for the latest fluoroquinolones.About:
This article is published in Biophysical Chemistry.The article was published on 2005-02-01. It has received 47 citations till now.read more
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Journal ArticleDOI
Molecular Basis of Enrofloxacin Translocation through OmpF, an Outer Membrane Channel of Escherichia coli - When Binding Does Not Imply Translocation
Kozhinjampara R. Mahendran,Eric Hajjar,Tivadar Mach,Marcos Lovelle,Amit Kumar,Isabel Sousa,Enrico Spiga,Helge Weingart,Paula Gameiro,Mathias Winterhalter,Matteo Ceccarelli +10 more
TL;DR: The results demonstrate how a single-point mutation of the porin, and the resulting intrachannel shift of the affinity site, may substantially modify translocation.
Journal ArticleDOI
Insights into the Environmental Resistance Gene Pool from the Genome Sequence of the Multidrug-Resistant Environmental Isolate Escherichia coli SMS-3-5
W. Florian Fricke,Meredith S. Wright,Angela H. Lindell,Derek M. Harkins,Craig Baker-Austin,Jacques Ravel,Ramunas Stepanauskas +6 more
TL;DR: The association of antimicrobial resistance with APEC virulence genes on pSMS35_130 highlights the risk of promoting the spread of virulence through the extensive use of antibiotics.
Journal ArticleDOI
Interactions of ciprofloxacin with DPPC and DPPG: fluorescence anisotropy, ATR-FTIR and 31P NMR spectroscopies and conformational analysis.
Hayet Bensikaddour,Karim Snoussi,Laurence Lins,Françoise Van Bambeke,Paul M. Tulkens,Robert Brasseur,Erik Goormaghtigh,Marie-Paule Mingeot-Leclercq +7 more
TL;DR: Comparing the binding of ciprofloxacin on DPPC and DPPG liposomes using quasi-elastic light scattering and steady-state fluorescence anisotropy demonstrated that the interactions of c iprofl oxacin with lipids depend markedly on the nature of their phosphate head groups and that ciprosatidylglycerol interacts preferentially with anionic lipid compounds present at a high content in these membranes.
Journal ArticleDOI
Mechanism for translocation of fluoroquinolones across lipid membranes.
Oana Cramariuc,Tomasz Róg,Matti Javanainen,Luca Monticelli,Luca Monticelli,A. V. Polishchuk,Ilpo Vattulainen,Ilpo Vattulainen +7 more
TL;DR: The findings propose a translocation mechanism in which zwitterionic CPFX molecules approach the membrane in stacks, but they diffuse through the membrane as neutral CPFX monomers due to intermolecular transfer of protons favored by partial solvation loss.
Journal ArticleDOI
Impact of Hapten Presentation on Antibody Binding at Lipid Membrane Interfaces
TL;DR: It is shown that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure, and it is demonstrated that ligand presentation strongly influences protein interactions with membrane-bound ligands.
References
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Journal ArticleDOI
Investigation of equilibria in solution. Determination of equilibrium constants with the HYPERQUAD suite of programs.
TL;DR: A new suite of 10 programs concerned with equilibrium constants and solution equilibria is described, which includes data preparation programs, pretreatment programs, equilibrium constant refinement and post-run analysis.
Book
Binding Constants: The Measurement of Molecular Complex Stability
TL;DR: This paper presents a method for quantifying theSolubility of Binding Constants using Optical Absorption Spectroscopy and Magnetic Resonance spectroscopy, and its applications in medicine and materials science.
Journal ArticleDOI
Drug-protein binding studies new trends in analytical and experimental methodology
TL;DR: This review will be focussed particularly on recent trends in the development of drug-protein binding methods including stereoselective and non-stereoselected aspects using chromatography, capillary electrophoresis and microdialysis as compared to the "conventional approach" using equilibrium dialysis, ultrafiltration or size exclusion chromatography.
Journal ArticleDOI
Photophysics and photochemistry of fluoroquinolones
Angelo Albini,Sandra Monti +1 more
TL;DR: Both activation of oxygen and various degradation pathways have been identified and the effects of medium and structure have been rationalized and can help in the understanding of the photochemistry occurring in biological environments and in the assessing of the correlation between structural characteristics and biological photodamage.
Journal ArticleDOI
Differences in susceptibility to quinolones of outer membrane mutants of Salmonella typhimurium and Escherichia coli.
TL;DR: The mechanism of penetration of quinolones through the bacterial outer membrane was studied and indicated that the lipopolysaccharide layer might form a permeability barrier for hydrophobic quInolones such as nalidixic acid but not forhydrophilic quinolate such as norfloxacin and ciprofloxACin.