Is It Time to Start Transitioning From 2D to 3D Cell Culture
Caleb Jensen,Yong Teng +1 more
TLDR
3D cellculture has the potential to provide alternative ways to study organ behavior via the use of organoids and is expected to eventually bridge the gap between 2D cell culture and animal models.Abstract:
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D) cell culturing techniques suggest compelling evidence that much more advanced experiments can be performed yielding valuable insights. When performing 3D cell culture experiments, the cell environment can be manipulated to mimic that of a cell in vivo and provide more accurate data about cell-to-cell interactions, tumor characteristics, drug discovery, metabolic profiling, stem cell research, and other types of diseases. Scaffold based techniques such as hydrogel-based support, polymeric hard material-based support, hydrophilic glass fiber, and organoids are employed, and each provide their own advantages and applications. Likewise, there are also scaffold free techniques used such as hanging drop microplates, magnetic levitation, and spheroid microplates with ultra-low attachment coating. 3D cell culture has the potential to provide alternative ways to study organ behavior via the use of organoids and is expected to eventually bridge the gap between 2D cell culture and animal models. The present review compares 2D cell culture to 3D cell culture, provides the details surrounding the different 3D culture techniques, as well as focuses on the present and future applications of 3D cell culture.read more
Citations
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3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions
João Rodrigues,João Rodrigues,Marcel A. Heinrich,Liliana Moreira Teixeira,Liliana Moreira Teixeira,Jai Prakash +5 more
TL;DR: An overview of state-of-the-art 3D models for studying tumor-stroma interactions, with a focus on understanding why the TME is a key target in cancer therapy.
Journal ArticleDOI
A guide to the organ-on-a-chip
Chak Ming Leung,Pim de Haan,Kacey Ronaldson-Bouchard,Ge-Ah Kim,Jihoon Ko,Hoon Suk Rho,Zhu Chen,Pamela Habibovic,Noo Li Jeon,Shuichi Takayama,Michael Shur,Gordana Vunjak-Novakovic,Olivier Frey,Elisabeth Verpoorte,Yi-Chin Toh +14 more
TL;DR: This Primer is intended to give an introduction to the aspects of OoC that need to be considered when developing an application- specific OoC, as well as subsequent assaying techniques to extract biological information from OoC devices.
Journal ArticleDOI
A guide to the organ-on-a-chip
TL;DR: Organs-on-chips (OoCs) as mentioned in this paper are systems containing engineered or natural miniature tissues grown inside microfluidic chips, which are designed to control cell microenvironments and maintain tissue-specific functions.
Journal ArticleDOI
Advances in Engineering Human Tissue Models.
TL;DR: In this paper, a review of 3D biomimetic cultures is presented, focusing on the technological bricks available to develop more physiologically relevant in vitro models of human tissues, including scaffold- or hydrogel-based formats, organotypic cultures, and organs-on-chips.
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