Journal ArticleDOI
Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2.
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TLDR
It is shown that the major lipopeptide product of BP-KJS-2 is the surfactin isoform, and that this lipopePTide showed strong antimicrobial activity against bacteria at the level of 0.05 mg/mL.Abstract:
Bacillus polyfermenticus KJS-2 (BP-KJS-2) was used to produce a lipopeptide-type surfactin. To accomplish this, a surfactin-producing BP-KJS-2 was fermented by soybeans. The surfactin was then purified by a procedure including ethanol treatment and preparative chromatography. Next, the biochemical structure of the purified surfactin was analyzed by electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI Q-Tof mass spectrometry (Q-Tof MS). In addition, the masses of the four peaks were determined to be 1007, 1021, 1035, and 1049 m/z revealing that the compound was mixture with quasi-molecular ions. Taken together, these findings indicated that the lipopeptide had a cyclic structure and amino acid composition of Gln-Leu-Leu-Leu-Val-Asp-Leu-Leu, and that the major lipopeptide product of BP-KJS-2 is the surfactin isoform. In addition, this lipopeptide showed strong antimicrobial activity against bacteria at the level of 0.05 mg/mL.read more
Citations
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Journal ArticleDOI
Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics
TL;DR: This review gives a detailed overview of the versatile functions of lipopeptides in the biology of Pseudomonas and Bacillus species, and highlights their role in competitive interactions with coexisting organisms, including bacteria, fungi, oomycetes, protozoa, nematodes and plants.
Journal ArticleDOI
Antimicrobial peptides of the genus Bacillus: a new era for antibiotics.
TL;DR: This work focuses on Bacillus-derived AMPs as a novel alternative approach to antibacterial drug development and provides an overview of the biosynthesis, mechanisms of action, applications, and effectiveness of different AMPs produced by members of the Bacillus genus.
Journal ArticleDOI
Microbial biofilms: biosurfactants as antibiofilm agents
TL;DR: Parts of biofilm characteristics are explored and the contribution of biologically derived surface-active agents (biosurfactants) to the disruption or inhibition of microbial biofilms are examined.
Journal Article
Surfactin – A Review on Biosynthesis, Fermentation, Purification and Applications
TL;DR: Current state of knowledge on biosynthesis of surfactin, its fermentative production, purification, analytical methods and biomedical applications is presented.
Journal ArticleDOI
Lipopeptide surfactants: Production, recovery and pore forming capacity.
Mnif Inès,Ghribi Dhouha +1 more
TL;DR: The main factors encountering lipopeptides production are presented along with the techniques developed for their extraction and purification and their ability to form pores and destabilize biological membrane permitting their use as antimicrobial, hemolytic and antitumor agents are discussed.
References
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Journal ArticleDOI
Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge.
Joachim Vater,Bärbel Kablitz,Christopher Wilde,Peter Franke,Neena Mehta,Swaranjit Singh Cameotra +5 more
TL;DR: An innovative method was developed for rapid sensitive detection and efficient structural characterization of lipopeptide biosurfactants by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry by using whole microbial cells and crude culture filtrates as targets in combination with surface tension measurements.
Journal ArticleDOI
Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis.
TL;DR: It is suggested that JH642, a strain which is not a producer of surfactin (genotypically sfp0), contains at least some of the genes encoding surfactIn production, and the sfp locus alters the transcriptional regulation of srf inJH642 cells.
Journal ArticleDOI
Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26.
TL;DR: The objective is to isolate an antagonist for use in the biological control of phytopathogenic fungi including Colletotrichum gloeosporioides, then to purify and characterize the biocontrol agent produced by the antagonist.
Journal ArticleDOI
Microbial Surfactants: A Review
K.K. Gautam,V.K. Tyagi +1 more
TL;DR: Biosurfactants are amphiphilic compounds produced on living surfaces, mostly microbial cell surface or excreted extracellularly and contain hydrophobic and hydrophilic moieties, that reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively.
Journal ArticleDOI
Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment
TL;DR: Biosurfactant may be produced with this organism using non-hydrocarbon substrates such as glucose and soybean that are readily available and would not require extensive purification for use in food and pharmaceutical industries.