Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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Ocular delivery of sunitinib-loaded nanoparticles doped in tragacanthic acid hydrogel in treatment of diabetic retinopathy in rats
Sana Pirmardvand Chegini,Jaleh Varshosaz,Alireza Dehghani,Mohsen Minaiyan,Hamid Mirmohammad Sadeghi +4 more
TL;DR: Sunitinib nanoparticles doped in TA hydrogel may be an appropriate substitution of bevacizumab in the treatment of DR.
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En-Face Optical Coherence Tomography Angiography for Longitudinal Monitoring of Retinal Injury
TL;DR: It is demonstrated that the en-face OCTA system is capable of visualizing capillary networks (~5 μm) and the corresponding tissue remodeling and growth dynamics allowing for separating acute injury from CNV.
Journal ArticleDOI
Long Non-Coding RNA PNKY Modulates the Development of Choroidal Neovascularization
TL;DR: This study suggests that the lncPNKY/PTBP1–miR-124 axis is involved in regulating the development of C NV, providing a potential therapeutic target for the treatment of CNV.
Journal ArticleDOI
Subretinal injection in mice to study retinal physiology and disease
Peirong Huang,Siddharth Narendran,Felipe Pereira,Shinichi Fukuda,Yosuke Nagasaka,Ivana Apicella,Praveen Yerramothu,Kenneth M. Marion,Xiaoyu Cai,Srinivas R. Sadda,Bradley D. Gelfand,Jayakrishna Ambati +11 more
TL;DR: A standardized protocol for SRI is proposed with 'procedure success' defined by follow-up examination of the retina and the retinal pigmented epithelium rather than solely via intraoperative endpoints, and it is found that trained ophthalmologists achieve reliable proficiency in this technique after ~350 practice injections.
Journal ArticleDOI
Metabolic Characterization of Ocular Tissues in Relation to Laser-Induced Choroidal Neovascularization in Rats.
Pinghui Wei,Meiqin He,Guoge Han +2 more
TL;DR: In this paper , the authors determined to assess the metabolomic alterations in a choroidal neovascularization (CNV) rat model to provide an insight into its pathogenesis.
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Prevalence of agerelated macular degeneration in the united states
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