Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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Retinal microglia: Just bystander or target for therapy?
TL;DR: It is concluded that this resident immune cell of the retina cannot be simply regarded as bystander of disease but may instead be a potential therapeutic target to be modulated in the treatment of degenerative and inflammatory diseases ofThe retina.
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Consensus guidelines for the use and interpretation of angiogenesis assays
Patrycja Nowak-Sliwinska,Kari Alitalo,Elizabeth Allen,Andrey Anisimov,Alfred C. Aplin,Robert Auerbach,Hellmut G. Augustin,Hellmut G. Augustin,David O. Bates,Judy R. van Beijnum,R. Hugh F. Bender,Gabriele Bergers,Gabriele Bergers,Andreas Bikfalvi,Joyce Bischoff,Barbara C. Böck,Barbara C. Böck,Peter C. Brooks,Federico Bussolino,Bertan Cakir,Peter Carmeliet,Daniel Castranova,Anca Maria Cimpean,Ondine Cleaver,George Coukos,George E. Davis,Michele De Palma,Anna Dimberg,Ruud P.M. Dings,Valentin Djonov,Andrew C. Dudley,Neil Dufton,Sarah-Maria Fendt,Napoleone Ferrara,Marcus Fruttiger,Dai Fukumura,Bart Ghesquière,Bart Ghesquière,Yan Gong,Robert J. Griffin,Adrian L. Harris,Christopher C.W. Hughes,Nan W. Hultgren,M. Luisa Iruela-Arispe,Melita Irving,Rakesh K. Jain,Raghu Kalluri,Joanna Kalucka,Robert S. Kerbel,Jan Kitajewski,Ingeborg Klaassen,Hynda K. Kleinmann,Pieter Koolwijk,Elisabeth Kuczynski,Brenda R. Kwak,Koen Marien,Juan M. Melero-Martin,Lance L. Munn,Roberto F. Nicosia,Agnès Noël,Jussi Nurro,Anna-Karin Olsson,Tatiana V. Petrova,Kristian Pietras,Roberto Pili,Jeffrey W. Pollard,Mark J. Post,Paul H.A. Quax,Gabriel A. Rabinovich,Marius Raica,Anna M. Randi,Domenico Ribatti,Curzio Rüegg,Reinier O. Schlingemann,Reinier O. Schlingemann,Stefan Schulte-Merker,Lois E.H. Smith,Jonathan W. Song,Steven A. Stacker,Jimmy Stalin,Amber N. Stratman,Maureen Van de Velde,Victor W.M. van Hinsbergh,Peter B. Vermeulen,Johannes Waltenberger,Brant M. Weinstein,Hong Xin,Bahar Yetkin-Arik,Seppo Ylä-Herttuala,Mervin C. Yoder,Arjan W. Griffioen +90 more
TL;DR: In vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis are described and critical aspects that are relevant for their execution and proper interpretation are highlighted.
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YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation
Jongshin Kim,Yoo Hyung Kim,Jaeryung Kim,Do Young Park,Hosung Bae,Da Hye Lee,Kyun Hoo Kim,Seon Pyo Hong,Seung Pil Jang,Yoshiaki Kubota,Young Guen Kwon,Dae-Sik Lim,Gou Young Koh +12 more
TL;DR: Results show that YAP/TAZ plays multifaceted roles for EC behaviors, proliferation, junction assembly, and metabolism in sprouting angiogenesis and barrier formation and maturation and could be a potential therapeutic target for treating neovascular diseases.
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Microglia in Retinal Degeneration.
TL;DR: This review provides an overview of recent studies that depict microglial responses in diverse retinal pathologies that have degeneration and chronic immune reactions as key pathophysiological components and discusses innovative immunomodulatory therapy strategies that dampen the detrimental immunological responses to improve disease outcome.
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Contribution of microglia-mediated neuroinflammation to retinal degenerative diseases.
TL;DR: An overview of the roles of microglia-mediated neuroinflammation in major retinal degenerative diseases like glaucoma, age-related macular degeneration, and diabetic retinopathy is given.
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Benoît Detry,Silvia Blacher,Charlotte Erpicum,Jenny Paupert,Ludovic Maertens,Catherine Maillard,Carine Munaut,Nor Eddine Sounni,Vincent Lambert,Jean-Michel Foidart,Jean-Marie Rakic,Didier Cataldo,Agnès Noël +12 more
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Mice without uPA, tPA, or plasminogen genes are resistant to experimental choroidal neovascularization.
Jean-Marie Rakic,Vincent Lambert,Carine Munaut,Khalid Bajou,Karine Peyrollier,Marie-Luz Alvarez-Gonzalez,Peter Carmeliet,Jean-Michel Foidart,Agnès Noël +8 more
TL;DR: It is indicated that choroidal neovascularization is extremely sensitive to the modulation of Plg/PA system activity and may provide a new strategy for the treatment of exudative AMD.
Journal ArticleDOI
Choroidal neovascularization is provided by bone marrow cells.
Minoru Tomita,Haruhiko Yamada,Yasushi Adachi,Yunze Cui,E. Yamada,Akiko Higuchi,Keizo Minamino,Yasuhiko Suzuki,Miyo Matsumura,Susumu Ikehara +9 more
TL;DR: Findings indicate that newly developed blood vessels in the CNV are derived from the BMCs and suggest that the inhibition of EPC mobilization from the bone marrow to the eyes could be a new approach to the fundamental treatment of CNV in ARMD.
Journal ArticleDOI
Anti-angiogenic therapy of exudative age-related macular degeneration: current progress and emerging concepts
TL;DR: The importance of putative molecular targets other than VEGF that might have been underestimated are considered, as well as emerging cellular mechanisms offer additional opportunities for innovative therapeutic approaches.