Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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A Novel “Inside‐Out” Intraocular Nanomedicine Delivery Mode for Nanomaterials’ Biological Effect Enhanced Choroidal Neovascularization Occlusion and Microenvironment Regulation
Yingying Jin,Yishun Guo,Jianhua Yang,Xiaoying Chu,Xiaoming Huang,Qingying Wang,Yanlin Zeng,Lili Su,Si Lu,Chenyang Wang,Jie Yang,Jia Qu,Yi Yang,Bailiang Wang +13 more
TL;DR: In this article , Pt nanoparticles (NPs) and Au NPs decorated zeolitic imidazolate framework 8 nanoplatform is developed to load indocyanine green for precise photodynamic therapy and microenvironment amelioration, which can penetrate the internal limiting membrane through Müller cells endocytosis and target to choroidal neovascularization (CNV) after intravitreal injection.
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A novel bispecific molecule delivered by recombinant AAV2 suppresses ocular inflammation and choroidal neovascularization.
TL;DR: The results suggest that gene therapy targeting VEGF and complement components could provide an innovative and long‐term strategy for ocular inflammatory and neovascular diseases.
Soluble Epoxide Hydrolase Inhibition for Ocular Diseases: Vision for the Future
Bomina Park,Timothy W. Corson +1 more
TL;DR: A recent mini-review as mentioned in this paper discusses the mounting evidence associating sEH with ocular diseases and its therapeutic value as a target, including the possibility of local delivery of inhibitors makes the eye an appealing target for future sEH drug development initiatives.
Journal ArticleDOI
Mobile Laser Indirect Ophthalmoscope: For the Induction of Choroidal Neovascularization in a Mouse Model.
Dov Weinberger,Elite Bor-Shavit,Tilda Barliya,Mor Dahbash,Opher Kinrot,Dan D. Gaton,Yael Nisgav,Tami Livnat +7 more
TL;DR: The mobile laser indirect ophthalmoscope can serve as a feasible and a reliable alternative method for the CNV induction in a mouse model and was successfully inhibited by both anti-human and mouse VEGF antibodies.
Journal ArticleDOI
Sodium Butyrate Inhibits Neovascularization Partially via TNXIP/VEGFR2 Pathway.
TL;DR: It is argued that NaBu inhibited neovascularization partially through TXNIP-regulated VEGFR2 signal pathway, which regulated the expression of VEGF receptor 2 in HUVEC cells was regulated byTXNIP undergoing NaBu treatment.
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TL;DR: In this article, the prevalence and distribution of age-related macular degeneration (AMD) in the United States by age, race/ethnicity, and gender was estimated.
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Jeffrey S. Heier,David M. Brown,Victor Chong,Jean-François Korobelnik,Peter K. Kaiser,Quan Dong Nguyen,Bernd Kirchhof,Allen C. Ho,Yuichiro Ogura,George D. Yancopoulos,Neil Stahl,Robert Vitti,Alyson J. Berliner,Yuhwen Soo,Majid Anderesi,Georg Groetzbach,Bernd Sommerauer,Rupert Sandbrink,Rupert Sandbrink,Christian Simader,Ursula Schmidt-Erfurth +20 more
TL;DR: It is demonstrated that aflibercept is an effective treatment for AMD, with the every-2-month regimen offering the potential to reduce the risk from monthly intravitreal injections and the burden of monthly monitoring.
Prevalence of agerelated macular degeneration in the united states
TL;DR: Age-related macular degeneration was far more prevalent among white than among black persons, and the number of persons having AMD will increase by 50% to 2.95 million in 2020.