Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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Journal ArticleDOI
Natural product inhibitors of ocular angiogenesis.
TL;DR: The multifunctional curcumin and the chalcone isoliquiritigenin have demonstrated promising anti-angiogenic effects in mouse models of DR and choroidal neovascularization (CNV) respectively, and the homoisoflavanone cremastranone and the flavonoid deguelin have been shown to inhibit ocular neov vascularization in more than one disease model.
Journal ArticleDOI
An Eye on Age-Related Macular Degeneration: The Role of MicroRNAs in Disease Pathology.
TL;DR: Before meaningful strides can be made to develop miRNAs as a diagnostic or therapeutic tool, reproducible miRNA profiles need to be established for the various clinical outcomes of AMD.
Journal ArticleDOI
Optimization of an Image-Guided Laser-Induced Choroidal Neovascularization Model in Mice
Yan Gong,Jie Li,Ye Sun,Zhongjie Fu,Chi-Hsiu Liu,Lucy P. Evans,Katherine Tian,Nicholas Saba,Thomas Fredrick,Peyton Morss,Jing Chen,Lois E.H. Smith +11 more
TL;DR: Details of laser-induced CNV are optimized using the image-guided laser photocoagulation system and dietary intake of omega-3 long-chain polyunsaturated fatty acid reduced laser- induced CNV in mice, suggesting that mice at 6-8 weeks of age are ideal for the laser- induces CNV model.
Journal ArticleDOI
Interferon-beta signaling in retinal mononuclear phagocytes attenuates pathological neovascularization.
TL;DR: The results reveal a protective role of Ifnar signaling in retinal immune homeostasis and highlight a potential use for IFN‐β therapy in the eye to limit chronic inflammation and pathological angiogenesis in AMD.
Journal ArticleDOI
Reliability of the mouse model of choroidal neovascularization induced by laser photocoagulation.
Stephen Poor,Yubin Qiu,Elizabeth Fassbender,Siyuan Shen,Amber Woolfenden,Andrea Delpero,Yong Kim,Natasha Buchanan,Thomas C. Gebuhr,Shawn Hanks,Erik Meredith,Bruce D Jaffee,Thaddeus P. Dryja +12 more
TL;DR: The recommended methods for conducting experiments with the mouse laser-induced CNV model are presented to enhance reproducibility and minimize investigator bias.
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Prevalence of agerelated macular degeneration in the united states
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