Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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Exercise alleviates neovascular age-related macular degeneration by inhibiting AIM2 inflammasome in myeloid cells.
B. Cui,Xu-Guang Guo,Wei Zhou,Xiaodan Zhang,Kai He,Ting Bai,Dongxue Lin,Yan Zhao,Shengnan Liu,Huiyang Zhou,Qing Wang,Xueming Yao,Ying Shi,Ruotian Xie,Xue Dong,Yiyang Lei,Mei Du,Yongsheng Chang,Heping Xu,Dongming Zhou,Ying Yu,Xiaohong Wang,Hua Yan +22 more
TL;DR: In this article , the AIM2 inflammasome and its product IL-1β were used as potential targets for treating age-related macular degeneration (nvAMD) patients.
Journal ArticleDOI
A hypoxia-regulated retinal pigment epithelium-specific gene therapy vector reduces choroidal neovascularization in a mouse model.
TL;DR: Hypoxia regulated, RPE-specific AAV vector-mediated sFlt-1 gene is a hypoxia-regulated antiangiogenic vector for wAMD by inhibiting VEGF-induced angiogenesis.
Journal ArticleDOI
Delayed regression of laser‐induced choroidal neovascularization in TNFα‐null mice
Hiroki Iwanishi,Osamu Yamanaka,Takayoshi Sumioka,Shingo Yasuda,Masayasu Miyajima,Shizuya Saika +5 more
TL;DR: TNFα gene knockout delays the regression of laser‐induced CNV in mice, and the mechanism underlying the phenotype might include the augmentation of neutrophil population in the treated tissue and attenuation of vascular endothelial cell apoptosis.
Journal ArticleDOI
A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway.
TL;DR: In this article, the antiangiogenic effects of peptide H-KI20 and illustrated signaling mechanisms were analyzed in human retinal microvascular endothelial cells (HRECs).
Journal ArticleDOI
Luteolin inhibits subretinal fibrosis and epithelial-mesenchymal transition in laser-induced mouse model via suppression of Smad2/3 and YAP signaling.
Chaoyang Zhang,Yao Zhang,Xin Hu,Zhenzhen Zhao,Zi-Yan Chen,Xi Wang,Zhi-Hong Zhang,Haiying Jin,Jingfa Zhang +8 more
TL;DR: In this article , the potential effects of luteolin on subretinal fibrosis and epithelial-mesenchymal transition (EMT) were investigated both in vivo and in vitro.
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TL;DR: In this article, the prevalence and distribution of age-related macular degeneration (AMD) in the United States by age, race/ethnicity, and gender was estimated.
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Jeffrey S. Heier,David M. Brown,Victor Chong,Jean-François Korobelnik,Peter K. Kaiser,Quan Dong Nguyen,Bernd Kirchhof,Allen C. Ho,Yuichiro Ogura,George D. Yancopoulos,Neil Stahl,Robert Vitti,Alyson J. Berliner,Yuhwen Soo,Majid Anderesi,Georg Groetzbach,Bernd Sommerauer,Rupert Sandbrink,Rupert Sandbrink,Christian Simader,Ursula Schmidt-Erfurth +20 more
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Prevalence of agerelated macular degeneration in the united states
TL;DR: Age-related macular degeneration was far more prevalent among white than among black persons, and the number of persons having AMD will increase by 50% to 2.95 million in 2020.