Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice.
Vincent Lambert,Julie Lecomte,Sylvain Hansen,Silvia Blacher,Maria-Luz Alvarez Gonzalez,Ingrid Struman,Nor Eddine Sounni,Eric Rozet,Pascal De Tullio,Jean-Michel Foidart,Jean-Marie Rakic,Agnès Noël +11 more
TLDR
This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments.Abstract:
The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7-14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.read more
Citations
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Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation.
TL;DR: Findings indicate that PGRN deficiency in Iba-1+ cells activates the lysosomal function that then leads to abnormal inflammation, which might promote the progression of the CNV.
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LXHY formula inhibits choroidal neovascularization development via inhibiting the recruitment and adhesion of BMCs to the retina
TL;DR: This experiment indicated that LXHY formula could inhibit CNV formation and development, probably by inhibiting the recruitment and attachment of BMCs into CNV area.
Journal ArticleDOI
Long-term fundus fluorescence angiography and real-time diagnosis of retinal diseases in non-human primate-animal models
Miaomiao Tang,Lu Zhang,Bin Song,Xiaoyuan Ji,Chenyu Wang,Houyu Wang,Hua Xu,Yuanyuan Su,Yuanyuan Su,Yao He +9 more
TL;DR: Wang et al. as discussed by the authors demonstrate that long-term fluorescence imaging of retinal vessels is enabled through a kind of fluorescent nanoagents, which is made of small-sized (hydrodynamic diameter: ∼ 3 nm) silicon nanoparticles (SiNPs) featuring strong fluorescence, robust photostability, lengthened blood residency and negligible toxicity.
Journal ArticleDOI
Voluntary Exercise Suppresses Choroidal Neovascularization in Mice.
Ryan D. Makin,Dionne Argyle,Shuichiro Hirahara,Yosuke Nagasaka,Mei Zhang,Zhen Yan,Nagaraj Kerur,Jayakrishna Ambati,Bradley D. Gelfand +8 more
TL;DR: These studies indicate that exercise before laser treatment is required to improve CNV outcomes, and provide the first experimental evidence that voluntary exercise improves C NV outcomes.
Journal Article
Apolipoprotein M Inhibits Angiogenic and Inflammatory Response by Sphingosine 1-Phosphate on Retinal Pigment Epithelium Cells
TL;DR: The present results provide a novel potential therapeutic target for AMD, and demonstrate a suppressive role for ApoM and S1P in the pathology of CNV progression, using a laser-induced CNV murine model.
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TL;DR: In this article, the prevalence and distribution of age-related macular degeneration (AMD) in the United States by age, race/ethnicity, and gender was estimated.
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Prevalence of agerelated macular degeneration in the united states
TL;DR: Age-related macular degeneration was far more prevalent among white than among black persons, and the number of persons having AMD will increase by 50% to 2.95 million in 2020.