Journal ArticleDOI
Metabolomic Assessment of the Effect of Dietary Cholesterol in the Progressive Development of Fatty Liver Disease
Maria Vinaixa,Miguel A. Rodríguez,Anna Rull,Raúl Beltrán,Cinta Bladé,Jesus Brezmes,Nicolau Cañellas,Jorge Joven,Xavier Correig +8 more
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TLDR
Dietary cholesterol is a causal factor in the development of both liver steatosis and hepatic inflammation, and (1)H NMR metabolomics is used for quantitative profiling of liver extracts from LDLr(-/-) mice.Abstract:
Nonalcoholic fatty liver disease is considered to be the hepatic manifestation of metabolic syndrome and is usually related to high-fat, high-cholesterol diets. With the rationale that the identification and quantification of metabolites in different metabolic pathways may facilitate the discovery of clinically accessible biomarkers, we report the use of (1)H NMR metabolomics for quantitative profiling of liver extracts from LDLr(-/-) mice, a well-documented mouse model of fatty liver disease. A total of 55 metabolites were identified, and multivariate analyses in a diet- and time-comparative strategy were performed. Dietary cholesterol increased the hepatic concentrations of cholesterol, triglycerides, and oleic acid but also decreased the [PUFA/MUFA] ratio as well as the relative amount of long-chain polyunsaturated fatty acids in the liver. This was also accompanied by variations of the hepatic concentration of taurine, glutathione, methionine, and carnitine. Heat-map correlation analyses demonstrated that hepatic inflammation and development of steatosis correlated with cholesterol and triglyceride NMR derived signals, respectively. We conclude that dietary cholesterol is a causal factor in the development of both liver steatosis and hepatic inflammation.read more
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Journal ArticleDOI
Uptake and Accumulation of Polystyrene Microplastics in Zebrafish (Danio rerio) and Toxic Effects in Liver.
TL;DR: The uptake and tissue accumulation of polystyrene microplastics in zebrafish were detected, and the toxic effects in liver were investigated, showing that both 5 μm and 70 nm PS-MPs caused inflammation and lipid accumulation in fish liver.
Journal ArticleDOI
The metabolomic window into hepatobiliary disease
Diren Beyoğlu,Jeffrey R. Idle +1 more
TL;DR: The metabolomic window into hepatobiliary disease sheds new light on the systems pathology of the liver.
Journal ArticleDOI
Metabolomics in Toxicology: Preclinical and Clinical Applications
TL;DR: This review focuses on the biomedical applications of metabolomic technology with emphasis on new analytical approaches, preclinical, and clinical applications of the technology, particularly those areas relevant to practicing toxicologists.
Journal ArticleDOI
Breast milk nutrient content and infancy growth.
Philippa Prentice,Ken K. Ong,Marieke H. Schoemaker,Eric A.F. van Tol,Jacques Vervoort,Ieuan A. Hughes,Carlo L. Acerini,David B. Dunger +7 more
TL;DR: Benefits of human breast milk in avoiding rapid infancy weight gain and later obesity could relate to its nutrient content, and the hypothesis that differential HM total calorie content or macronutrient contents may be associated with infancy growth was tested.
Journal ArticleDOI
Non-alcoholic fatty liver disease: A sign of systemic disease
Isabella Reccia,Jayant Kumar,Cherif Akladios,Francesco Virdis,Madhava Pai,Nagy A. Habib,Duncan Spalding +6 more
TL;DR: This review brings together the current understanding of the pathogenesis that makes NAFLD a systemic disease and identifies several mechanisms involved in its pathogenesis and progression.
References
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Journal ArticleDOI
Steatohepatitis: a tale of two "hits"?
Journal ArticleDOI
Glutathione Metabolism and Its Implications for Health
TL;DR: Animal and human studies demonstrate that adequate protein nutrition is crucial for the maintenance of GSH homeostasis, and compelling evidence shows that GSH synthesis is regulated primarily by gamma-glutamylcysteine synthetase activity, cysteine availability, and GSH feedback inhibition.
Journal ArticleDOI
Nonalcoholic steatohepatitis: Summary of an AASLD Single Topic Conference
Brent A. Neuschwander-Tetri,Brent A. Neuschwander-Tetri,Stephen H. Caldwell,Stephen H. Caldwell +3 more
TL;DR: The research agenda for the future includes establishing the role of insulin resistance and abnormal lipoprotein metabolism in NASH, determining the pathogenesis of cellular injury, defining predisposing genetic abnormalities, identifying better noninvasive predictors of disease, and defining effective therapy.
Journal ArticleDOI
Molecular mediators of hepatic steatosis and liver injury
TL;DR: Recent advances in the understanding of the molecular events contributing to hepatic steatosis and nonalcoholic steatohepatitis are highlighted.
Journal ArticleDOI
Obesity and Nonalcoholic Fatty Liver Disease: Biochemical, Metabolic and Clinical Implications
TL;DR: It is not clear whether NAFLD causes metabolic dysfunction or whether metabolic dysfunction is responsible for IHTG accumulation, or possibly both, but it is likely that abnormalities in fatty acid metabolism are key factors involved in the development of insulin resistance, dyslipidemia, and other cardiometabolic risk factors associated withNAFLD.
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