Journal ArticleDOI
Methicillin-resistant Staphylococcus aureus typing methods: which should be the international standard?
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TLDR
The majority of phenotypic and genotypic techniques developed for MRSA are not sufficiently discriminatory, reproducible, stable or useful in an outbreak to be acceptable.About:
This article is published in Journal of Hospital Infection.The article was published on 2000-03-01. It has received 106 citations till now. The article focuses on the topics: Typing & Antibacterial agent.read more
Citations
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Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology
Sahreena Lakhundi,Kunyan Zhang +1 more
TL;DR: The origin of MRSA is described, with emphasis on the diverse nature of staphylococcal cassette chromosome mec (SCCmec).
Journal ArticleDOI
Guidelines for the validation and application of typing methods for use in bacterial epidemiology
A. van Belkum,Panayotis T. Tassios,Lenie Dijkshoorn,Sara Hæggman,Barry Cookson,Norman K. Fry,V. Fussing,Jonathan Green,Edward J. Feil,Peter Gerner-Smidt,Sylvain Brisse,Marc Struelens +11 more
TL;DR: Newer and older, phenotypic and genotypic methods for typing of all clinically relevant bacterial species are described according to their principles, advantages and disadvantages and Criteria for their evaluation and application and the interpretation of their results are proposed.
Journal ArticleDOI
Co-evolutionary aspects of human colonisation and infection by Staphylococcus aureus.
Alex van Belkum,Damian C. Melles,Jan L. Nouwen,Willem B. van Leeuwen,Willem J. B. van Wamel,Margreet C. Vos,Heiman F. L. Wertheim,Henri A. Verbrugh +7 more
TL;DR: The present review paper will explore the relevance of a large variety of staphylococcal virulence and colonization factors have been identified as well as a number of host' colonisation and infection susceptibility traits and other environmental factors that define the colonisation or infection state in humans.
Journal ArticleDOI
Multilocus sequence typing and the evolution of methicillin-resistant Staphylococcus aureus
D. A. Robinson,Mark C. Enright +1 more
TL;DR: This review summarises recent studies that have elucidated the evolutionary history of methicillin-resistant Staphylococcus aureus and demonstrates that community-acquired MRSA strains are typically related only distantly to hospitalMRSA strains, and thus represent novel acquisitions of SCCmec.
Journal ArticleDOI
Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary.
Ferenc Peles,Martin Wagner,L. Varga,Ingeborg Hein,Petra Rieck,K. Gutser,Péter Keresztúri,Gábor Kardos,I. Turcsányi,Béla Béri,András Szabó +10 more
TL;DR: The PFGE patterns showed genetic relatedness between the S. aureus strains recovered from quarter milk and bulk milk on two large farms, implying that on farms having a high number of mastitic cows, S.Aureus from infected udders may contaminate bulk milk and, subsequently, raw milk products.
References
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Journal ArticleDOI
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
Fred C. Tenover,Robert D. Arbeit,Richard V. Goering,P. A. Mickelsen,Barbara E Murray,D. H. Persing,B. Swaminathan +6 more
TL;DR: This research presents a novel, scalable and scalable approach that allows for real-time assessment of the severity of the infection and its impact on patients’ health.
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Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.
Paul R. Hunter,M A Gaston +1 more
TL;DR: An index of discrimination for typing methods is described, based on the probability of two unrelated strains being characterized as the same type, which may be used to compare typing methods and select the most discriminatory system.
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Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
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Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility.
TL;DR: Therapy was resumed with the com -bination of arbekacin and ampicillin/sulbactam which has been shown to have synergic activity against MRSA.