Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Book ChapterDOI
Biotechnological Approaches in Propagation and Improvement of Withania somnifera (L.) Dunal
Kulwinder Kaur,Pritika Singh,Rupam Guleri,Baldev Singh,Kuldeep Kaur,Varinder Singh,Pratap Kumar Pati +6 more
TL;DR: The present chapter briefly discusses the challenges in W. somnifera and presents a quick overview of biotechnological advances to address these challenges and highlights the futuristic approaches that would lay a foundation in the conglomeration of W. SomniferA as an ideal model medicinal plant.
Journal ArticleDOI
In vitro Shoot multiplication of Stevia rebaudiana, an important Plant with high Economic and Medicinal values
Navin Pradhan,Padmanabh Dwivedi +1 more
TL;DR: This micro-propagation method using half-strength MS media could be economical and effectively used for mass production of Stevia rebaudiana under in vitro condition.
Study of Genetic Transformation of Medicinal Plants, Withania Somnifera (L.) Dunal By Agrobacterium Tumefaciens (MTCC-431)
TL;DR: Standardize protocol for genetic transformation in a important medicinal plant will be useful for the enhancement of increased production of bioactive metabolites.
Journal ArticleDOI
Optimization of growth regulators and explant source for micropropagation and cost effective ex vitro rooting in Poshita Winter Cherry (Withania somnifera L.)
Ajit Arun Waman,B. N. Sathyanarayana,K. Umesha,Balakrishna Gowda,T. H. Ashok,M. Rajesh,R. G. Guruprakash +6 more
TL;DR: Results revealed that shoot induction was possible only after an intervening callus phase, irrespective of the concentration of growth regulators present in the nutrient pool, and the present protocol can be exploited on a commercial scale to obtain maximum benefits from the improved cultivar.
Multiple shoot regeneration in clerodendrum incisum l., - an ornamental woody shrub
TL;DR: A rapid and efficient protocol for induction of multiple shoots from nodal explants of an ornamental woody shrub was developed and plantlets were established in soil as phenotypically normal mature plants.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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