Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
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TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Journal ArticleDOI
Rapid Micropropagation of Withania somnifera L. Accessions from Axillary Meristems
Farzana Sabir,Neelam S. Sangwan,Narayan D. Chaurasiya,Laxmi N. Misra,Rakesh Tuli,Rajender S. Sangwan +5 more
TL;DR: Stable production of withanolides from in vitro regenerated shoots was comparable to the yields from field grown mother plants, indicating the in vitro methodology could be used successfully for the true-to-type plant regeneration of Withania somnifera accessions.
Journal ArticleDOI
Addressing Challenges to Enhance the Bioactives of Withania somnifera through Organ, Tissue, and Cell Culture Based Approaches
Pritika Singh,Rupam Guleri,Amrita Angurala,Kuldeep Kaur,Kulwinder Kaur,Sunil C. Kaul,Renu Wadhwa,Pratap Kumar Pati +7 more
TL;DR: The potential of in vitro cell/organ cultures for the production of therapeutically valuable compounds and their large-scale production in bioreactors has received significant attention in recent years.
Journal ArticleDOI
Effect of nitrogen and carbon sources on in vitro shoot multiplication, root induction and withanolides content in Withania somnifera (L.) Dunal
TL;DR: The present study resulted in the production of 4.42-fold higher multiple shoots and 2.6-foldHigher roots as well as enhancing the contents of all major withanolides in regenerated plants when compared to previous reports on W. somnifera regeneration.
Journal ArticleDOI
In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal
Rajangam Udayakumar,Chang Won Choi,K T Kim,Sei Chang Kim,S. Kasthurirengan,Thankaraj Salammal Mariashibu,J J Sahaya Rayan,Andy Ganapathi +7 more
TL;DR: The protocol developed in this study offers a simple and improved in vitro method to regenerate W. somnifera (L.) and yields a higher number of shoots within a short period.
Book ChapterDOI
Phytochemical Genomics of Ashwagandha
TL;DR: Emerging metabolomic area like phytochemical genomics has also been applied in case of Withania somnifera for metabolite detection and its structural validation and emerging metabolomics resources have provided leads to study intra and inter-specific gene diversity.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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