Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
More filters
Journal ArticleDOI
Critical factors influencing in vitro propagation and modulation of important secondary metabolites in Withania somnifera (L.) dunal
TL;DR: In this article , a review on the advancements and prospects in biotechnological interventions to meet the worldwide demands for W. somnifera and its bioactive compounds is presented.
Journal ArticleDOI
Withania somnifera (Ashwagandha): Regeneration through Meristem Culture
TL;DR: High frequency of callus induction was achieved from shoot tip explants of Withania somnifera (Hindi name-Ashwagandha) as compared to leaf explants on MS medium supplemented with IAA and Kinetin, and it was found suitable for complete plant regeneration through meristem culture.
Journal ArticleDOI
In vitro mass multiplication and estimation of camptothecin (CPT) in Ophiorrhiza mungos L. var. angustifolia (Thw.) Hook. f.
TL;DR: The results suggest the possibility of using in vitro regenerated plants as a possible alternative source for CPT without harming the natural population.
Book ChapterDOI
Morphogenesis, Genetic Stability, and Secondary Metabolite Production in Untransformed and Transformed Cultures
Mihir Halder,Sumita Jha +1 more
TL;DR: This review attempts to highlight an overall knowledge about the role of differentiation and morphogenesis on SM production with genetic and biochemical stability of commonly used different in vitro cultures.
Journal ArticleDOI
Effect of Hydrothermal Time, pH, and Alkali on Crystal Phase and Morphology of Manganese Oxides
Zhiqing Zhang,Jin Mu +1 more
TL;DR: In this paper, the effect of pH, pH, and alkali on morphology and composition of manganese oxides was investigated, and the results of XRD, TEM, and SEM showed that the product was pure γ-MnOOH when using NaOH in the system, but a mixture of Mn3O4 and γ•MnO4 was obtained when using NH3·-H2O.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more