Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Book ChapterDOI
Regeneration from Nodal Explants of Field-Grown Litchi ( Litchi chinensis Sonn.) Fruit Trees
TL;DR: A research protocol is highlighted which addresses the technical inputs for reproducible and efficient method of in vitro regeneration of elite litchi trees appropriate for clonal propagation and has been proven advantageous to the horticulturists and the industry for recalcitrant trees.
Journal IssueDOI
A Note on the Micropropagation of Endangered Medicinal Plant, Withania Somnifera
TL;DR: Micropropagation of Withania somnifera (Solanaceae) collected locally was tried, which has tremendous potential in the use of several ailments such as Parkinson’s, memory loss and heart diseases, but over use is making this plant disappear fast.
Rapid callus induction and regenerative studies in shoot tip explants of Withania somnifera (L.) dunal-indian ginseng
TL;DR: The efficient large scale in vitro regeneration protocol was developed by indirect organogenesis of Withania somnifera (L.) Dunal by direct organogenesis and transfer of shoots derived from callus in primary cultures to proliferation medium of different cytokinins.
Book ChapterDOI
Regulation of In Vitro Morphogenesis by Modulation of Culture Conditions in Withania somnifera L. Using Cotyledonary Node Explants
Nigar Fatima,Mohammad Anis +1 more
TL;DR: In this paper, an in vitro propagation of cotyledonary node segments (CN) attained from 7-day-old aseptic seedlings in a highly valuable medicinal plant (Withania somnifera L. L. was performed from explants as bared to diverse range of cytokinins viz; 6-benzyl adenine (BA), kinetin (Kn), or 2-isopentenyl adeniine (2iP) individually or in combined arrangement with various auxins namely indole 3-acetic
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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