Journal ArticleDOI
miR156 modulates rhizosphere acidification in response to phosphate limitation in Arabidopsis.
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TLDR
The results suggest that miR156 regulates the process of rhizosphere acidification in plants, which may account for the decreased H+-ATPase activity in 35S:MIM156 plants.Abstract:
Rhizosphere acidification is a general response to Pi deficiency, especially in dicotyledonous plants. However, the signaling pathway underlying this process is still unclear. Here, we demonstrate that miR156 is induced in the shoots and roots of wild type Arabidopsis plants during Pi starvation. The rhizosphere acidification capacity was increased in 35S:MIR156 (miR156 overexpression) plants, but was completely inhibited in 35S:MIM156 (target mimicry) plants. Both 35S:MIR156 and 35S:MIM156 plants showed altered proton efflux and H(+)-ATPase activity. In addition, significant up-regulation of H(+)-ATPase activity in 35S:MIR156 roots coupled with increased citric acid and malic acid exudates was observed. qRT-PCR results showed that most H(+)-ATPase and PPCK gene transcript levels were decreased in 35S:MIM156 plants, which may account for the decreased H(+)-ATPase activity in 35S:MIM156 plants. MiR156 also affect the root architecture system. Collectively, our results suggest that miR156 regulates the process of rhizosphere acidification in plants.read more
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Journal ArticleDOI
An extended root phenotype : the rhizosphere, its formation and impacts on plant fitness
Carla de la Fuente Cantó,Marie Simonin,Marie Simonin,Marie Simonin,Eoghan King,Lionel Moulin,Malcolm J. Bennett,Gabriel Castrillo,Laurent Laplaze +8 more
TL;DR: Current understanding of how plants shape the rhizosphere is reviewed and how applying their solutions in crops will enable us to harvest the benefits of the extended root phenotype is discussed.
Journal ArticleDOI
Phytochrome-interacting factors directly suppress MIR156 expression to enhance shade-avoidance syndrome in Arabidopsis
TL;DR: It is shown that phytochrome-interacting factors, which are regulated in a light-dependent manner, directly repress MIR156 genes and promote the expression of SPL genes to enhance shade-avoidance responses.
Journal ArticleDOI
H2A.Z promotes the transcription of MIR156A and MIR156C in Arabidopsis by facilitating the deposition of H3K4me3
TL;DR: SWR1-C component mutations demonstrate that H2A.Z contributes to the high expression of MIR156A/MIR156C early in shoot development, but does not regulate the timing of vegetative phase change.
Journal ArticleDOI
Effect of phosphate on the adsorption of antibiotics onto iron oxide minerals: Comparison between tetracycline and ciprofloxacin.
Yuwei Zhu,Qingxin Yang,Taotao Lu,Taotao Lu,Wei Qi,Haojing Zhang,Mengjie Wang,Zhichong Qi,Zhichong Qi,Weifeng Chen +9 more
TL;DR: The results highlight the importance of phosphate in exploring the environmental fate of antibiotics in natural environment by investigating the effects of phosphate on the adsorption behaviors of tetracycline and ciprofloxacin in soil environments.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
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Book ChapterDOI
Assay of inorganic phosphate, total phosphate and phosphatase
TL;DR: The method is about seven times as sensitive as the Fiske–SubbaRow procedure and involves less pipetting, but it is not very satisfactory for determining inorganic phosphate if labile phosphate esters are present in large excess.
Journal ArticleDOI
Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource
TL;DR: Physiological, biochemical, and molecular studies of white lupin and other species response to P-deficiency have identified targets that may be useful for plant improvement, and Genomic approaches involving identification of expressed sequence tags found under low-P stress may also yield target sites for plant improved.
Journal ArticleDOI
Prediction of Plant MicroRNA Targets
Matthew W. Rhoades,Brenda J. Reinhart,Lee P. Lim,Christopher B. Burge,Bonnie Bartel,David P. Bartel +5 more
TL;DR: This work predicts regulatory targets for 14 Arabidopsis microRNAs (miRNAs) by identifying mRNAs with near complementarity and identifies members of transcription factor gene families involved in developmental patterning or cell differentiation.