Journal ArticleDOI
Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane
Gen He,Ning Hu,Alexander M. Xu,Alexander M. Xu,Xiangling Li,Yunlong Zhao,Yunlong Zhao,Xi Xie +7 more
TLDR
This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array‐based systems that are of crucial importance for the development of future practical biomedical platforms.Abstract:
Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long‐term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array‐based systems that are of crucial importance for the development of future practical biomedical platforms.read more
Citations
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Nanowire-Mediated Delivery Enables Functional Interrogation of Primary Immune Cells: Application to the Analysis of Chronic Lymphocytic Leukemia
Alex K. Shalek,Jellert T. Gaublomme,Lili Wang,Nir Yosef,Nicolas Chevrier,Mette S. Andersen,Jacob T. Robinson,Nathalie Pochet,Donna Neuberg,Rona S. Gertner,Ido Amit,Jennifer R. Brown,Nir Hacohen,Aviv Regev,Aviv Regev,Catherine J. Wu,Hongkun Park +16 more
TL;DR: It is demonstrated that vertical silicon nanowires enable gene-specific manipulation of diverse murine and human immune cells with negligible toxicity and suggest a functional classification that can potentially guide the selection of patient-specific therapies in CLL and highlight the opportunities for nanotechnology to drive biological inquiry.
Journal ArticleDOI
PEDOT:PSS-Based Bioelectronic Devices for Recording and Modulation of Electrophysiological and Biochemical Cell Signals
TL;DR: A survey of recent trends in polyethylenedioxythiophene (PEDOT:PSS) electrophysiological sensors highlights the potential of this multifunctional material to revolve current technology and to enable longlasting, multichannel polymer probes as discussed by the authors.
Journal ArticleDOI
High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.
Justin Brooks,Grayson Minnick,Prithvijit Mukherjee,Arian Jaberi,Lingqian Chang,Horacio D. Espinosa,Ruiguo Yang +6 more
TL;DR: This review strives to examine a variety of methods for in vitro and ex vivo intracellular delivery such as flow-through microfluidics, engineered substrates, and automated probe-based systems from the perspective of throughput and control.
Journal ArticleDOI
Tutorial: using nanoneedles for intracellular delivery.
Ciro Chiappini,Ciro Chiappini,Chen Yaping,Stella Aslanoglou,Stella Aslanoglou,Stella Aslanoglou,Anna Mariano,Valentina Mollo,Huanwen Mu,Enrica De Rosa,Gen He,Ennio Tasciotti,Xi Xie,Francesca Santoro,Wenting Zhao,Nicolas H. Voelcker,Roey Elnathan,Roey Elnathan +17 more
TL;DR: In this paper, the authors present a tutorial to equip researchers with the knowledge required to develop a nano-injection workflow, including the selection of nanoneedle devices, approaches for cargo loading and strategies for interfacing to biological systems.
Journal ArticleDOI
Non-viral transfection technologies for next-generation therapeutic T cell engineering
TL;DR: In this paper, the authors provide an in-depth discussion on the avid search for alternatives to viral vectors, discusses key considerations for T cell engineering technologies, and provides an overview of the emerging spectrum of non-viral transfection technologies for T cells.
References
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Therapeutic Nanoparticles for Drug Delivery in Cancer
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