Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization
Ciro Chiappini,E. De Rosa,Jonathan O. Martinez,Xuewu Liu,Joseph A. M. Steele,Molly M. Stevens,Ennio Tasciotti +6 more
Reads0
Chats0
TLDR
It is shown that a tunable array of biodegradable nan oneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.Abstract:
The controlled delivery of nucleic acids to selected tissues remains an inefficient process mired by low transfection efficacy, poor scalability because of varying efficiency with cell type and location, and questionable safety as a result of toxicity issues arising from the typical materials and procedures employed. High efficiency and minimal toxicity in vitro has been shown for intracellular delivery of nuclei acids by using nanoneedles, yet extending these characteristics to in vivo delivery has been difficult, as current interfacing strategies rely on complex equipment or active cell internalization through prolonged interfacing. Here, we show that a tunable array of biodegradable nanoneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.read more
Citations
More filters
Journal ArticleDOI
Diverse Applications of Nanomedicine
Beatriz Pelaz,Christoph Alexiou,Ramon A. Alvarez-Puebla,Frauke Alves,Frauke Alves,Anne M. Andrews,Sumaira Ashraf,Lajos P. Balogh,Laura Ballerini,Alessandra Bestetti,Cornelia Brendel,Susanna Bosi,Mónica Carril,Warren C. W. Chan,Chunying Chen,Xiaodong Chen,Xiaoyuan Chen,Zhen Cheng,Daxiang Cui,Jianzhong Du,Christian Dullin,Alberto Escudero,Alberto Escudero,Neus Feliu,Mingyuan Gao,Michael D. George,Yury Gogotsi,Arnold Grünweller,Zhongwei Gu,Naomi J. Halas,Norbert Hampp,Roland K. Hartmann,Mark C. Hersam,Patrick Hunziker,Ji Jian,Xingyu Jiang,Philipp Jungebluth,Pranav Kadhiresan,Kazunori Kataoka,Ali Khademhosseini,Jindřich Kopeček,Nicholas A. Kotov,Harald F. Krug,Dong Soo Lee,Claus-Michael Lehr,Kam W. Leong,Xing-Jie Liang,Mei Ling Lim,Luis M. Liz-Marzán,Xiaowei Ma,Paolo Macchiarini,Huan Meng,Helmuth Möhwald,Paul Mulvaney,Andre E. Nel,Shuming Nie,Peter Nordlander,Teruo Okano,Jose Oliveira,Tai Hyun Park,Reginald M. Penner,Maurizio Prato,Maurizio Prato,Víctor F. Puntes,Vincent M. Rotello,Amila Samarakoon,Raymond E. Schaak,Youqing Shen,Sebastian Sjöqvist,Andre G. Skirtach,Andre G. Skirtach,Mahmoud Soliman,Molly M. Stevens,Hsing-Wen Sung,Ben Zhong Tang,Rainer Tietze,Buddhisha Udugama,J. Scott VanEpps,Tanja Weil,Tanja Weil,Paul S. Weiss,Itamar Willner,Yuzhou Wu,Yuzhou Wu,Lily Yang,Zhao Yue,Qian Zhang,Qiang Zhang,Xian-En Zhang,Yuliang Zhao,Xin Zhou,Wolfgang J. Parak +91 more
TL;DR: An overview of recent developments in nanomedicine is provided and the current challenges and upcoming opportunities for the field are highlighted and translation to the clinic is highlighted.
Journal ArticleDOI
Enhanced Cancer Immunotherapy by Microneedle Patch-Assisted Delivery of Anti-PD1 Antibody
TL;DR: It is found that a single administration of the MN patch induces robust immune responses in a B16F10 mouse melanoma model compared to MN without degradation trigger or intratumoral injection of free aPD1 with the same dose.
Journal ArticleDOI
Advanced Soft Materials, Sensor Integrations, and Applications of Wearable Flexible Hybrid Electronics in Healthcare, Energy, and Environment.
Hyo-Ryoung Lim,Hee Seok Kim,Raza Qazi,Raza Qazi,Young-Tae Kwon,Jae-Woong Jeong,Woon-Hong Yeo +6 more
TL;DR: An all-inclusive review of the newly developed WFHE along with a summary of imperative requirements of material properties, sensor capabilities, electronics performance, and skin integrations is provided.
Journal ArticleDOI
Degradability and Clearance of Silicon, Organosilica, Silsesquioxane, Silica Mixed Oxide, and Mesoporous Silica Nanoparticles.
TL;DR: The degradability and clearance timelines of various siliceous nanomaterials are compared and it is highlighted that researchers can select a specific nanommaterial in this large family according to the targeted applications and the required clearance kinetics.
Journal ArticleDOI
Drug delivery systems and materials for wound healing applications.
Saghi Saghazadeh,Chiara Rinoldi,Maik Schot,Sara Saheb Kashaf,Fatemeh Sharifi,Elmira Jalilian,Kristo Nuutila,Giorgio Giatsidis,Pooria Mostafalu,Hossein Derakhshandeh,Kan Yue,Wojciech Swieszkowski,Adnan Memic,Ali Tamayol,Ali Khademhosseini +14 more
TL;DR: The pathophysiology of chronic wounds is discussed and then the materials used for engineering drug delivery systems are discussed, and the architecture of the delivery platform and its ability to modulate drug delivery are discussed.
References
More filters
Journal ArticleDOI
Vascular-specific growth factors and blood vessel formation
George D. Yancopoulos,Samuel Davis,Nicholas W. Gale,John S. Rudge,Stanley J. Wiegand,Jocelyn Holash +5 more
TL;DR: New findings in newly discovered vascular growth factors demand re-evaluation of therapeutic efforts aimed at regulating blood vessel growth in ischaemia, cancer and other pathological settings.
Journal ArticleDOI
Knocking down barriers: advances in siRNA delivery
TL;DR: An update on the progress of RNAi therapeutics is provided and novel synthetic materials for the encapsulation and intracellular delivery of nucleic acids are highlighted.
Journal ArticleDOI
Growth factors and cytokines in wound healing.
TL;DR: A review of the specific roles of these growth factors and cytokines during wound healing can be found in this article, where patients are treated by three growth factors: PDGF-BB, bFGF, and GM-CSF.
Journal ArticleDOI
Progress and problems with the use of viral vectors for gene therapy
TL;DR: With the development of a leukaemia-like syndrome in two patients cured of a disease by gene therapy, it is timely to contemplate how far this technology has come, and how far it still has to go.
Journal ArticleDOI
Synthetic DNA delivery systems.
Dan Luo,Saltzman Wm +1 more
TL;DR: The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology and rapid advances have recently been made in understanding of mechanisms for DNA stability and transport within cells.