Newcastle disease virus (NDV)-based assay demonstrates interferon-antagonist activity for the NDV V protein and the Nipah virus V, W, and C proteins.
Man Seong Park,Megan L. Shaw,Jorge L. Muñoz-Jordán,Jérôme Cros,Takaaki Nakaya,Nicole M. Bouvier,Peter Palese,Adolfo García-Sastre,Christopher F. Basler +8 more
TLDR
It is shown that expression of the NDV V protein or the Nipah virus V, W, or C proteins rescues NDV-GFP replication in the face of the transfection-induced IFN response, and that the NDVs could be used to screen proteins expressed from plasmids for the ability to counteract the host cellIFN response.Abstract:
We have generated a recombinant Newcastle disease virus (NDV) that expresses the green fluorescence protein (GFP) in infected chicken embryo fibroblasts (CEFs). This virus is interferon (IFN) sensitive, and pretreatment of cells with chicken alpha/beta IFN (IFN-α/β) completely blocks viral GFP expression. Prior transfection of plasmid DNA induces an IFN response in CEFs and blocks NDV-GFP replication. However, transfection of known inhibitors of the IFN-α/β system, including the influenza A virus NS1 protein and the Ebola virus VP35 protein, restores NDV-GFP replication. We therefore conclude that the NDV-GFP virus could be used to screen proteins expressed from plasmids for the ability to counteract the host cell IFN response. Using this system, we show that expression of the NDV V protein or the Nipah virus V, W, or C proteins rescues NDV-GFP replication in the face of the transfection-induced IFN response. The V and W proteins of Nipah virus, a highly lethal pathogen in humans, also block activation of an IFN-inducible promoter in primate cells. Interestingly, the amino-terminal region of the Nipah virus V protein, which is identical to the amino terminus of Nipah virus W, is sufficient to exert the IFN-antagonist activity. In contrast, the anti-IFN activity of the NDV V protein appears to be located in the carboxy-terminal region of the protein, a region implicated in the IFN-antagonist activity exhibited by the V proteins of mumps virus and human parainfluenza virus type 2.read more
Citations
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Journal ArticleDOI
Region of Nipah virus C protein responsible for shuttling between the cytoplasm and nucleus.
TL;DR: Results indicate that the IFN-antagonist activity of NiV-C occurs in the cytoplasm, and indicates that the nuclear export determinant is not a classical CRM1-dependent nuclear export signal.
Book ChapterDOI
Newcastle Disease Virus
TL;DR: Newcastle disease virus (NDV) is the aetiological agent of one of the most prevalent and virulent avian diseases in the world, and causes millions of birds to die, and significantly affects the poultry industries of many countries in Africa, Asia and South America.
Book ChapterDOI
Avian immunosuppressive diseases and immune evasion
TL;DR: In this article , the effects on the innate and acquired immune responses and the mechanisms by which mycotoxins, stress, and infectious agents cause immunosuppression are discussed.
Journal ArticleDOI
La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation.
Giuseppe Pisanelli,Maudry Laurent-Rolle,Balaji Manicassamy,Alan Belicha-Villanueva,Juliet Morrison,Bernardo Lozano-Dubernard,Felipa Castro-Peralta,Giuseppe Iovane,Adolfo García-Sastre +8 more
TL;DR: It is demonstrated that LPMV-V protein antagonizes type I but not type II IFN signaling by binding STAT2, a component of the type I IFN cascade, and prevents the IFN-induced phosphorylation and nuclear translocation of STAT1 and STAT2 thereby inhibiting cellular responses to IFN α/β.
Book ChapterDOI
Nipah and Hendra Viruses
TL;DR: It is presumed that horses and pigs that have acted as an intermediary host to humans had been infected by indirect contact with pteropid bats endemic in these regions, although this has not been experimentally proven.
References
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Efficient selection for high-expression transfectants with a novel eukaryotic vector
TL;DR: The results showed that high concentrations of G418 efficiently yielded L cell and CHO cell transfectants stably producing IL-2 at levels comparable with those previously attained using gene amplification.
Journal ArticleDOI
Nipah Virus: A Recently Emergent Deadly Paramyxovirus
Kaw Bing Chua,William J. Bellini,Paul A. Rota,Brian H. Harcourt,Azaibi Tamin,Sai Kit Lam,Thomas G. Ksiazek,Pierre E. Rollin,Sherif R. Zaki,Wun-Ju Shieh,Cynthia S. Goldsmith,Duane J. Gubler,John T. Roehrig,Bryan T. Eaton,A. R. Gould,James G. Olson,P. Daniels,Ai Ee Ling,Clarence J. Peters,Larry J. Anderson,Brian W. J. Mahy +20 more
TL;DR: Electron microscopic, serologic, and genetic studies indicate that the Nipah virus belongs to the family Paramyxoviridae and is most closely related to the recently discovered Hendra virus, and it is suggested that these two viruses are representative of a new genus within the familyparamyxviridae.
Journal ArticleDOI
Influenza A Virus Lacking the NS1 Gene Replicates in Interferon-Deficient Systems
Adolfo García-Sastre,Andrej Egorov,Demetrius Matassov,Sabine Brandt,David E. Levy,Joan E. Durbin,Peter Palese,Thomas Muster +7 more
TL;DR: In this paper, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated through the use of reverse genetics, and it has been shown that the NS 1 protein plays a crucial role in inhibiting interferon-mediated antiviral responses of the host.
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