Newcastle disease virus (NDV)-based assay demonstrates interferon-antagonist activity for the NDV V protein and the Nipah virus V, W, and C proteins.
Man Seong Park,Megan L. Shaw,Jorge L. Muñoz-Jordán,Jérôme Cros,Takaaki Nakaya,Nicole M. Bouvier,Peter Palese,Adolfo García-Sastre,Christopher F. Basler +8 more
TLDR
It is shown that expression of the NDV V protein or the Nipah virus V, W, or C proteins rescues NDV-GFP replication in the face of the transfection-induced IFN response, and that the NDVs could be used to screen proteins expressed from plasmids for the ability to counteract the host cellIFN response.Abstract:
We have generated a recombinant Newcastle disease virus (NDV) that expresses the green fluorescence protein (GFP) in infected chicken embryo fibroblasts (CEFs). This virus is interferon (IFN) sensitive, and pretreatment of cells with chicken alpha/beta IFN (IFN-α/β) completely blocks viral GFP expression. Prior transfection of plasmid DNA induces an IFN response in CEFs and blocks NDV-GFP replication. However, transfection of known inhibitors of the IFN-α/β system, including the influenza A virus NS1 protein and the Ebola virus VP35 protein, restores NDV-GFP replication. We therefore conclude that the NDV-GFP virus could be used to screen proteins expressed from plasmids for the ability to counteract the host cell IFN response. Using this system, we show that expression of the NDV V protein or the Nipah virus V, W, or C proteins rescues NDV-GFP replication in the face of the transfection-induced IFN response. The V and W proteins of Nipah virus, a highly lethal pathogen in humans, also block activation of an IFN-inducible promoter in primate cells. Interestingly, the amino-terminal region of the Nipah virus V protein, which is identical to the amino terminus of Nipah virus W, is sufficient to exert the IFN-antagonist activity. In contrast, the anti-IFN activity of the NDV V protein appears to be located in the carboxy-terminal region of the protein, a region implicated in the IFN-antagonist activity exhibited by the V proteins of mumps virus and human parainfluenza virus type 2.read more
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Inhibition of the host antiviral response by Nipah virus: Current understanding and future perspectives
TL;DR: This review examines the current data on inhibition of the host antiviral response for each of the NiV proteins gathered from viral protein expression systems, in vitro data using recombinant NiV mutants and from in vivo studies using recombination mutants, as well as a future perspective regarding the direction of the field.
Journal ArticleDOI
The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus.
Jin Il Kim,Jin Il Kim,Min Woong Hwang,Min Woong Hwang,Ilseob Lee,Ilseob Lee,Sehee Park,Sehee Park,Sangmoo Lee,Sangmoo Lee,Joon Yong Bae,Joon Yong Bae,Jun Heo,Donghwan Kim,Seok Il Jang,Mee Sook Park,Mee Sook Park,Hyung-Joo Kwon,Jin Won Song,Man Seong Park,Man Seong Park +20 more
TL;DR: Results suggest that the C-terminal PDZ-binding motif in the NS1 protein may determine viral persistence in the human and avian IAV interface.
Journal ArticleDOI
Identification of Optimal Insertion Site in Recombinant Newcastle Disease Virus (rNDV) Vector Expressing Foreign Gene to Enhance Its Anti-Tumor Effect
Ziye Pan,Jinjiao He,Lubna M. Rasoul,Yunye Liu,Ruixiang Che,Yun Ding,Xiaocheng Guo,Jiarui Yang,Dehua Zou,Hua Zhang,Deshan Li,Hongwei Cao +11 more
TL;DR: NP and P gene junction in r NDV is the optimal insertion site for foreign genes expression to enhance rNDV’s anti-tumor effects.
Journal ArticleDOI
Newcastle disease virus as a vaccine vector for sars-cov-2
Edris Shirvani,Siba K. Samal +1 more
TL;DR: Newcastle disease virus (NDV), an avian virus, has several well-suited properties for development of a vector vaccine against SARS-CoV-2, and this work elaborates on the idea of considering NDV as a vaccine vector for Sars-Cov-2.
Journal ArticleDOI
Molecular evolution and genetic variations of V and W proteins derived by RNA editing in Avian Paramyxoviruses
TL;DR: The molecular clock analysis revealed higher conservation of V protein sequence compared to W protein indicating the important role played by V protein in viral replication, pathogenesis and immune evasion and the estimates of synonymous and non-synonymous substitution rates suggested negative selection pressure on the V and W proteins within species indicating their low evolution rate.
References
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Efficient selection for high-expression transfectants with a novel eukaryotic vector
TL;DR: The results showed that high concentrations of G418 efficiently yielded L cell and CHO cell transfectants stably producing IL-2 at levels comparable with those previously attained using gene amplification.
Journal ArticleDOI
Nipah Virus: A Recently Emergent Deadly Paramyxovirus
Kaw Bing Chua,William J. Bellini,Paul A. Rota,Brian H. Harcourt,Azaibi Tamin,Sai Kit Lam,Thomas G. Ksiazek,Pierre E. Rollin,Sherif R. Zaki,Wun-Ju Shieh,Cynthia S. Goldsmith,Duane J. Gubler,John T. Roehrig,Bryan T. Eaton,A. R. Gould,James G. Olson,P. Daniels,Ai Ee Ling,Clarence J. Peters,Larry J. Anderson,Brian W. J. Mahy +20 more
TL;DR: Electron microscopic, serologic, and genetic studies indicate that the Nipah virus belongs to the family Paramyxoviridae and is most closely related to the recently discovered Hendra virus, and it is suggested that these two viruses are representative of a new genus within the familyparamyxviridae.
Journal ArticleDOI
Influenza A Virus Lacking the NS1 Gene Replicates in Interferon-Deficient Systems
Adolfo García-Sastre,Andrej Egorov,Demetrius Matassov,Sabine Brandt,David E. Levy,Joan E. Durbin,Peter Palese,Thomas Muster +7 more
TL;DR: In this paper, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated through the use of reverse genetics, and it has been shown that the NS 1 protein plays a crucial role in inhibiting interferon-mediated antiviral responses of the host.
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