Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
Reads0
Chats0
TLDR
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.Abstract:
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.read more
Citations
More filters
Journal ArticleDOI
Chemically-selective imaging of brain structures with CARS microscopy
TL;DR: The feasibility of in vivo CARS vibrational histology as a clinical tool for neuropathological diagnosis is illustrated by demonstrating the use of CARS microscopy in identifying normal brain structures and primary glioma in fresh unfixed and unstained ex vivo brain tissue.
Journal ArticleDOI
Zweiphotonenabsorption und das Design von Zweiphotonenfarbstoffen
Journal ArticleDOI
Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System†
TL;DR: Imidazoline-2-thione containing OCl(-) probes, PIS and NIS, are designed and prepared, which operate through specific reactions with O Cl(-) that yield corresponding fluorescent imidazolium ions, indicating that this probe could have wide biological applications.
Journal ArticleDOI
A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion
TL;DR: This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (Kd(TP)) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2-]m in a rat hippocampal slice at a depth of 100−200 μm without interference from other metal ions through the use of twophoton microscopy.
Journal ArticleDOI
Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique
TL;DR: It is shown that high quality three-dimensional imaging can be realized at depths beyond the reach of conventional multiphoton microscopy and adaptive optics methods, albeit over restricted distances for a given correction.
References
More filters
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Electromagnetic Diffraction in Optical Systems. II. Structure of the Image Field in an Aplanatic System
B. Richards,Emil Wolf +1 more
TL;DR: In this article, an investigation of the structure of the electromagnetic field near the focus of an aplanatic system which images a point source is made, and the results are illustrated by diagrams and in a tabulated form based on data obtained by extensive calculations on an electronic computor.
Journal ArticleDOI
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.