Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
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TLDR
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.Abstract:
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.read more
Citations
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Stochastic Optical Reconstruction Microscopy (STORM): A Method for Superresolution Fluorescence Imaging
TL;DR: Stochastic optical reconstruction microscopy (STORM) is described, a method for superresolution imaging based on the high accuracy localization of individual fluorophores: molecules that can be switched between a nonfluorescent and a fluorescent state by exposure to light.
An in vivo biosensor for neurotransmitter release and In situ receptor activity : acetylcholine and the M1 muscarinic receptor
TL;DR: It is found that chronic implantation of M1-CNiFERs in frontal cortex of the adult rat to elucidate the muscarinic action of the atypical neuroleptics clozapine and olanzapine found that these drugs potently inhibited in situMuscarinic receptor activity.
Journal ArticleDOI
The application of fluorescent quantum dots to confocal, multiphoton, and electron microscopic imaging.
TL;DR: This work has sought to characterize and exploit quantum-dot utility for enabling simultaneous multiprotein immunolabeling in fixed cells and tissues and for correlated light and electron microscopic analysis in highly correlated microscopies.
Journal ArticleDOI
Intravital microscopy: a novel tool to study cell biology in living animals.
TL;DR: The overall aim of this review is to give the reader a general idea of the potential applications of intravital microscopy with a particular emphasis on subcellular imaging, and some of the most exciting studies in this field will be presented using resolution as a main organizing criterion.
Journal ArticleDOI
Pig skin structure and transdermal delivery of liposomes: a two photon microscopy study.
TL;DR: Analysis of LAURDAN Generalized Polarization images of pig skin show that the canyons are filled with a non-polar poorly hydrated material, similar to that observed in pig skin stratum corneum, and these results together with the data obtained from skin autofluorescence images suggest that these canYons are invaginations/extension of SC material.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Electromagnetic Diffraction in Optical Systems. II. Structure of the Image Field in an Aplanatic System
B. Richards,Emil Wolf +1 more
TL;DR: In this article, an investigation of the structure of the electromagnetic field near the focus of an aplanatic system which images a point source is made, and the results are illustrated by diagrams and in a tabulated form based on data obtained by extensive calculations on an electronic computor.
Journal ArticleDOI
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.