Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
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TLDR
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.Abstract:
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.read more
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Journal ArticleDOI
Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine
Conor L. Evans,X. Sunney Xie +1 more
TL;DR: Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free imaging technique that is capable of real-time, nonperturbative examination of living cells and organisms based on molecular vibrational spectroscopy.
Journal ArticleDOI
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.
Hiroshi Hama,Hiroshi Kurokawa,Hiroyuki Kawano,Ryoko Ando,Tomomi Shimogori,Hisayori Noda,Kiyoko Fukami,Asako Sakaue-Sawano,Atsushi Miyawaki +8 more
TL;DR: In Scale-treated mouse brain, neurons labeled with genetically encoded fluorescent proteins were visualized at an unprecedented depth in millimeter-scale networks and at subcellular resolution, suggesting that the Scale method will be useful for light microscopy–based connectomics of cellular networks in brain and other tissues.
Journal ArticleDOI
Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
TL;DR: This Primer explains the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM, and demonstrates how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.
Journal ArticleDOI
Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience
Karel Svoboda,Ryohei Yasuda +1 more
TL;DR: The principles of 2PE microscopy are reviewed, recent applications are highlighted, its limitations are discussed, and areas for future research and development are pointed to.
Journal ArticleDOI
SERS--a single-molecule and nanoscale tool for bioanalytics.
TL;DR: This critical review introduces the physics behind single-molecule SERS and discusses the capabilities of the effect in bioanalytics and surface enhanced hyper-Raman scattering (SEHRS).
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Electromagnetic Diffraction in Optical Systems. II. Structure of the Image Field in an Aplanatic System
B. Richards,Emil Wolf +1 more
TL;DR: In this article, an investigation of the structure of the electromagnetic field near the focus of an aplanatic system which images a point source is made, and the results are illustrated by diagrams and in a tabulated form based on data obtained by extensive calculations on an electronic computor.
Journal ArticleDOI
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.