Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
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TLDR
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.Abstract:
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.read more
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High-Resolution Intravital Microscopy
Volker Andresen,Karolin Pollok,Karolin Pollok,Jan-Leo Rinnenthal,Jan-Leo Rinnenthal,Laura Oehme,Robert Günther,Heinrich Spiecker,Helena Radbruch,Helena Radbruch,Jenny Gerhard,Jenny Gerhard,Anje Sporbert,Zoltán Cseresnyés,Anja E. Hauser,Raluca Niesner,Raluca Niesner +16 more
TL;DR: Multi-beam striped-illumination is presented, for the first time, able to visualize the dynamic interactions between B cells and immune complex deposits on follicular dendritic cells within germinal centers of live mice, leading to affinity maturation of the humoral immune response.
Journal ArticleDOI
Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves
TL;DR: Using femtosecond 860 nm excitation pulses, fluorescence lifetimes can be measured in leaves of Arabidopsis thaliana and Alocasia wentii under excitation-annihilation free conditions, both for the F0- and the Fm-state.
Journal ArticleDOI
In Vivo Microscopy Reveals Extensive Embedding of Capillaries within the Sarcolemma of Skeletal Muscle Fibers
Brian Glancy,Li-Yueh Hsu,Lam Dao,Matthew Bakalar,Stephanie French,David J. Chess,Joni Taylor,Martin Picard,Angel Aponte,Mathew P. Daniels,Shervin Esfahani,Samuel W. Cushman,Robert S. Balaban +12 more
TL;DR: To provide insight into mitochondrial function in vivo, the 3D spatial relationship between capillaries, mitochondria, and muscle fibers in live mice is evaluated.
Journal ArticleDOI
Mapping of native inorganic elements and injected nanoparticles in a biological organ with laser-induced plasma
Vincent Motto-Ros,Lucie Sancey,Qianli Ma,François Lux,Xueshi Bai,X. C. Wang,Jin Yu,Gérard Panczer,Olivier Tillement +8 more
TL;DR: Emission spectroscopy of laser-induced plasma from a thin section of mouse kidney successfully detected inorganic elements, Na, Ca, Cu, and Gd, naturally contained in the organ or artificially injected in the form of Gd-based nanoparticle.
Journal ArticleDOI
Review of near-infrared methods for wound assessment
TL;DR: Technologies that employ near-infrared (NIR) light form a prominent contingent among the existing and emerging technologies and address clinical wound management needs along the entire healing trajectory of a wound.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Electromagnetic Diffraction in Optical Systems. II. Structure of the Image Field in an Aplanatic System
B. Richards,Emil Wolf +1 more
TL;DR: In this article, an investigation of the structure of the electromagnetic field near the focus of an aplanatic system which images a point source is made, and the results are illustrated by diagrams and in a tabulated form based on data obtained by extensive calculations on an electronic computor.
Journal ArticleDOI
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.