Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
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TLDR
Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.Abstract:
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.read more
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Journal ArticleDOI
Nitrogen and phosphorus co-doped graphene quantum dots: synthesis from adenosine triphosphate, optical properties, and cellular imaging
Arundithi Ananthanarayanan,Yue Wang,Parimal Routh,Mahasin Alam Sk,Aung Than,Ming Lin,Jie Zhang,Jie Chen,Handong Sun,Peng Chen +9 more
TL;DR: A simple strategy is demonstrated for the synthesis of nitrogen and phosphorus co-doped GQDs from a single biomolecule precursor (adenosine triphosphate - ATP) that exhibit high fluorescence quantum yield, strong two-photon upconversion, small molecular weight, high photostability, and good biocompatibility.
Journal ArticleDOI
Theranostic iridium(III) complexes as one- and two-photon phosphorescent trackers to monitor autophagic lysosomes.
Liang He,Cai-Ping Tan,Rui-Rong Ye,Yi-Zhi Zhao,Ya-Hong Liu,Qiang Zhao,Liang-Nian Ji,Zong-Wan Mao +7 more
TL;DR: It is demonstrated that LysoIr2 can effectively implement two functions, namely autophagy induction and lysosomal tracking, in the visualization of autophagosomal-lysosomal fusion.
Journal ArticleDOI
Metal Ion-Responsive Fluorescent Probes for Two-Photon Excitation Microscopy.
TL;DR: This review summarizes the challenges associated with the design of two-photon excitable fluorescent probes and labels and offers an overview on recent efforts in developing selective and sensitive reagents for the detection of metal ions in biological systems.
Journal ArticleDOI
Strong two-photon absorption in new asymmetrically substituted porphyrins: interference between charge-transfer and intermediate-resonance pathways.
Mikhail Drobizhev,Fanqing Meng,Aleksander Rebane,Yuriy Stepanenko,Eric Nickel,Charles W. Spangler +5 more
TL;DR: This is the first experimental observation of the combined effect of these two pathways on one particular two-photon transition, resulting in quantum-interference-modulated 2PA strength.
Journal ArticleDOI
Multi-photon excitation microscopy
Alberto Diaspro,Alberto Diaspro,Paolo Bianchini,Giuseppe Vicidomini,Mario Faretta,Paola Ramoino,Cesare Usai +6 more
TL;DR: 2PE implementations in noninvasive optical bioscopy or laser-based treatments point out to the relevance in clinical applications, and a list of potential applications and variations on the theme is given to offer a starting point for advancing new applications and developments.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Electromagnetic Diffraction in Optical Systems. II. Structure of the Image Field in an Aplanatic System
B. Richards,Emil Wolf +1 more
TL;DR: In this article, an investigation of the structure of the electromagnetic field near the focus of an aplanatic system which images a point source is made, and the results are illustrated by diagrams and in a tabulated form based on data obtained by extensive calculations on an electronic computor.
Journal ArticleDOI
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson,Warren R. Zipfel,Rebecca M. Williams,S. W. Clark,Marcel P. Bruchez,Frank W. Wise,Watt W. Webb +6 more
TL;DR: This work characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals and found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.