Pannexin 1 channels regulate leukocyte emigration through the venous endothelium during acute inflammation.
Alexander W. Lohman,Igor L. Leskov,Joshua T. Butcher,Scott R. Johnstone,Tara A. Stokes,Daniela Begandt,Leon J. DeLalio,Angela K. Best,Silvia Penuela,Norbert Leitinger,Kodi S. Ravichandran,Karen Y. Stokes,Brant E. Isakson +12 more
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TLDR
It is reported that the ATP-release channel Pannexin1 (Panx1) opens downstream of EC activation by TNF-α, placing Panx1 channels at the centre of cytokine crosstalk with purinergic signalling in the endothelium.Abstract:
Inflammatory cell recruitment to local sites of tissue injury and/or infection is controlled by a plethora of signalling processes influencing cell-to-cell interactions between the vascular endothelial cells (ECs) in post-capillary venules and circulating leukocytes. Recently, ATP-sensitive P2Y purinergic receptors have emerged as downstream regulators of EC activation in vascular inflammation. However, the mechanism(s) regulating cellular ATP release in this response remains elusive. Here we report that the ATP-release channel Pannexin1 (Panx1) opens downstream of EC activation by TNF-α. This process involves activation of type-1 TNF receptors, recruitment of Src family kinases (SFK) and SFK-dependent phosphorylation of Panx1. Using an inducible, EC-specific Panx1 knockout mouse line, we report a previously unidentified role for Panx1 channels in promoting leukocyte adhesion and emigration through the venous wall during acute systemic inflammation, placing Panx1 channels at the centre of cytokine crosstalk with purinergic signalling in the endothelium.read more
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Dissertation
Pannexin 1 Expression in Bovine Cumulus-Oocyte Complexes and Function During in vitro Maturation
TL;DR: It is suggested that the manuscript should be rewritten in a chapters-by- chapters format to facilitate more detailed discussion of the author's research and its aims and methods.
Journal ArticleDOI
Determination of structural features that underpin the pannexin1 channel inhibitory activity of the peptide 10Panx1.
Anne Caufriez,Arthur Lamouroux,Charlotte Martin,Debora Iaculli,Rafael Gozalbes,Maria D. Mayan,Brenda R. Kwak,Andrés Tabernilla,Mathieu Vinken,Steven Ballet +9 more
TL;DR: In this article , a structure-activity relationship study was performed to proteolytically stabilize the 10Panx1 sequence, and it was shown that the side chains of Gln3 and Asp8 are crucial for the channel inhibitory capacity.
Journal ArticleDOI
Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm
Lisa Maria Metz,T. Feige,Larissa de Biasi,Agnes Ehrenberg,Joscha Mulorz,Laura Mara Toska,Friedrich Reusswig,Christine Quast,Norbert Gerdes,Malte Kelm,Hubert Schelzig,Margitta Elvers +11 more
TL;DR: In this paper , the authors analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm and found that platelets are important for the migration of neutrophils into the abdominal wall induced by direct cell interaction and by activation of endothelial cells.
Journal ArticleDOI
High PANX1 Expression Leads to Neutrophil Recruitment and the Formation of a High Adenosine Immunosuppressive Tumor Microenvironment in Basal-like Breast Cancer
TL;DR: High PANX1 expression is associated with high TAN infiltration and adenosine production to induce local immunosuppression in basal-like breast cancer TME and TANs highly expressed ENTPD1 (CD39)/NT5E (CD73).
Evaluating the Effect of Silibinin on the Expression of Pannexin1 Gene During Hepatic Ischemia-Reperfusion
Hadis Musavi,Mohamad Sadegh Safaee,Zohreh Nasiri,Fateme Ghorbani,Parisa Mohamadi,Elham,Rostami,Abbas Khonakdar-Tarsi,Mobina Faghani Lor +8 more
TL;DR: Liver the Panx1 gene during I/R is probably one of the mechanisms of anti-inflammatory effects of silibinin.
References
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Book ChapterDOI
Receptors for Purines and Pyrimidines
Vera Ralevic,Geoffrey Burnstock +1 more
TL;DR: In this review particular emphasis is placed on the discrepancy between the concentrations ofadenosine, ADP, and ATP in the purine receptors of UDP and UTP.
Journal ArticleDOI
Getting to the site of inflammation: the leukocyte adhesion cascade updated
TL;DR: This Review focuses on new aspects of one of the central paradigms of inflammation and immunity — the leukocyte adhesion cascade.
Journal ArticleDOI
Nitric oxide: an endogenous modulator of leukocyte adhesion.
P Kubes,M Suzuki,D. N. Granger +2 more
TL;DR: Data suggest that endothelium-derived NO may be an important endogenous modulator of leukocyte adherence and that impairment of NO production results in a pattern ofLeukocyte adhesion and emigration that is characteristic of acute inflammation.
Journal ArticleDOI
Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor
TL;DR: Pannexin‐1, a recently described mammalian protein that functions as a hemichannel when ectopically expressed, is identified as this dye‐uptake pathway and signalling through pannexin•1 is required for processing of caspase‐1 and release of mature IL‐1β induced by P2X7 receptor activation.
Journal ArticleDOI
Intravascular danger signals guide neutrophils to sites of sterile inflammation.
Braedon McDonald,Keir Pittman,Gustavo B. Menezes,Simon A. Hirota,Ingrid Slaba,Christopher C. M. Waterhouse,Paul L. Beck,Daniel A. Muruve,Paul Kubes +8 more
TL;DR: Dynamic in vivo imaging revealed a multistep hierarchy of directional cues that guide neutrophil localization to sites of sterile inflammation, and the underlying mechanisms of recruitment of neutrophils into injured tissue.
Related Papers (5)
Pannexin 1 channels mediate ‘find-me’ signal release and membrane permeability during apoptosis
Faraaz B. Chekeni,Michael R. Elliott,Michael R. Elliott,Joanna K. Sandilos,Scott F. Walk,Scott F. Walk,Jason M. Kinchen,Jason M. Kinchen,Eduardo R. Lazarowski,Allison Armstrong,Allison Armstrong,Silvia Penuela,Dale W. Laird,Guy S. Salvesen,Brant E. Isakson,Douglas A. Bayliss,Kodi S. Ravichandran,Kodi S. Ravichandran +17 more