Pannexin 1 channels regulate leukocyte emigration through the venous endothelium during acute inflammation.
Alexander W. Lohman,Igor L. Leskov,Joshua T. Butcher,Scott R. Johnstone,Tara A. Stokes,Daniela Begandt,Leon J. DeLalio,Angela K. Best,Silvia Penuela,Norbert Leitinger,Kodi S. Ravichandran,Karen Y. Stokes,Brant E. Isakson +12 more
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TLDR
It is reported that the ATP-release channel Pannexin1 (Panx1) opens downstream of EC activation by TNF-α, placing Panx1 channels at the centre of cytokine crosstalk with purinergic signalling in the endothelium.Abstract:
Inflammatory cell recruitment to local sites of tissue injury and/or infection is controlled by a plethora of signalling processes influencing cell-to-cell interactions between the vascular endothelial cells (ECs) in post-capillary venules and circulating leukocytes. Recently, ATP-sensitive P2Y purinergic receptors have emerged as downstream regulators of EC activation in vascular inflammation. However, the mechanism(s) regulating cellular ATP release in this response remains elusive. Here we report that the ATP-release channel Pannexin1 (Panx1) opens downstream of EC activation by TNF-α. This process involves activation of type-1 TNF receptors, recruitment of Src family kinases (SFK) and SFK-dependent phosphorylation of Panx1. Using an inducible, EC-specific Panx1 knockout mouse line, we report a previously unidentified role for Panx1 channels in promoting leukocyte adhesion and emigration through the venous wall during acute systemic inflammation, placing Panx1 channels at the centre of cytokine crosstalk with purinergic signalling in the endothelium.read more
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Journal ArticleDOI
Purinergic signaling in nervous system health and disease: Focus on pannexin 1.
Juan C. Sanchez-Arias,Emma van der Slagt,Haley A. Vecchiarelli,Rebecca C. Candlish,Nicole York,Penelope A. Young,Olga Shevtsova,Afnan Juma,Marie-Ève Tremblay,Leigh Anne Swayne +9 more
TL;DR: The PANX1 ATP-release channel has been extensively studied in the literature as discussed by the authors, including the role of PANX 1 in neuronal development and plasticity, glial signaling, and neuron-glial-immune interactions.
Journal ArticleDOI
Dendritic Cell Migration Toward CCL21 Gradient Requires Functional Cx43.
Richard Ruez,Juan Dubrot,Alice Zoso,Marc Bacchetta,Filippo Molica,Stéphanie Hugues,Brenda R. Kwak,Marc Chanson +7 more
TL;DR: Examination of the CCR7-induced signaling pathways in BMDCs revealed normal receptor-induced mobilization of intracellular Ca2+.
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Extracellular ATP: A powerful inflammatory mediator in the central nervous system
TL;DR: In this article , the authors investigated the role of extracellular ATP (eATP) in the regulation of microglia, astroglia and oligodendroglia responses.
Journal ArticleDOI
Pannexins and Connexins: Their Relevance for Oocyte Developmental Competence
Paweł Kordowitzki,Gabriela Sokołowska,Marta Wasielak-Politowska,Agnieszka Skowronska,Mariusz T. Skowronski +4 more
TL;DR: The role of Pannexins and Connexins for the processes of oogenesis, folliculogenesis, oocyte maturation and fertilization will be discussed, and at the end of this review, Pannexin and Conclave related pathologies and their impact on the developmental competence of oocytes will be provided.
Journal ArticleDOI
Pannexin 1 mutation found in melanoma tumor reduces phosphorylation, glycosylation, and trafficking of the channel-forming protein.
Daniel Nouri-Nejad,Brooke L. O’Donnell,Chetan S. Patil,Rafael E Sanchez-Pupo,Danielle Johnston,Samar Sayedyahossein,Kristina Jurcic,Rebecca Lau,Laszlo Gyenis,David W. Litchfield,Michael F. Jackson,Gregory B. Gloor,Silvia Penuela +12 more
TL;DR: In this paper, the effect of single amino acid changes in PANX1 domains using naturally occurring variants reported in cancer patient tumors was investigated and a previously reported variant (Q5H) is present in cancer cells, but was not different from the wild type in glycosylation, trafficking or channel function and did not affect cellular properties.
References
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TL;DR: In this review particular emphasis is placed on the discrepancy between the concentrations ofadenosine, ADP, and ATP in the purine receptors of UDP and UTP.
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Journal ArticleDOI
Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor
TL;DR: Pannexin‐1, a recently described mammalian protein that functions as a hemichannel when ectopically expressed, is identified as this dye‐uptake pathway and signalling through pannexin•1 is required for processing of caspase‐1 and release of mature IL‐1β induced by P2X7 receptor activation.
Journal ArticleDOI
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TL;DR: Dynamic in vivo imaging revealed a multistep hierarchy of directional cues that guide neutrophil localization to sites of sterile inflammation, and the underlying mechanisms of recruitment of neutrophils into injured tissue.
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