PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.
TLDR
PTEN gene mutation and expression may play an important role in the occurrence and development of gastric cancer.Abstract:
AIM: To discuss the possible effect of PTEN gene mutations on occurrence and development of gastric cancer.
METHODS: Fifty-three gastric cancer specimens were selected to probe PTEN gene mutations in genome of gastric cancer and paracancerous tissues using PCR-SSCP-DNA sequencing method based on microdissection and to observe the protein expression by immunohistochemistry technique.
RESULTS: PCR-SSCP-DNA sequencing indicated that 4 kinds of mutation sites were found in 5 of 53 gastric cancer specimens. One kind of mutation was found in exons. AA-TCC mutation was located at 40bp upstream of 3’ lateral exon 7 (115946 AA-TCC). Such mutations led to terminator formation in the 297th codon of the PTEN gene. The other 3 kinds of mutation were found in introns, including a G-C point mutation at 91 bp upstream of 5’ lateral exon 5(90896 G-C), a T-G point mutation at 24 bp upstream of 5’ lateral exon 5 (90963 T-G), and a single base A mutation at 7 bp upstream of 5’ lateral exon 5 (90980 A del). The PTEN protein expression in gastric cancer and paracancerous tissues detected using immunohistochemistry technique indicated that the total positive rate of PTEN protein expression was 66% in gastric cancer tissue, which was significantly lower than that (100%) in paracancerous tissues (P < 0.005).
CONCLUSION: PTEN gene mutation and expression may play an important role in the occurrence and development of gastric cancer.read more
Citations
More filters
Journal ArticleDOI
Gastric cancer—molecular and clinical dimensions
TL;DR: Representative molecular and clinical dimensions of gastric cancer are reviewed and the future prospects are excellent for defining biomarker-based subsets of patients and application of specific therapeutics are excellent.
Journal ArticleDOI
Pathogenetic mechanisms in gastric cancer
Jing Shi,Yiping Qu,Peng Hou +2 more
TL;DR: The pathogenetic mechanisms of GC are the focus of this review and have opened unprecedented opportunities for better understanding of the molecular mechanisms of gastric carcinogenesis and the development of novel therapeutic strategies for this cancer.
Journal ArticleDOI
PTEN genomic deletions that characterize aggressive prostate cancer originate close to segmental duplications
Maisa Yoshimoto,Olga Ludkovski,Dave DeGrace,Julia L. Williams,Andrew Evans,Kanishka Sircar,Tarek A. Bismar,Paulo Nuin,Jeremy A. Squire +8 more
TL;DR: A meta‐analysis of 311 published human genome array datasets was conducted and determined that the minimal prostate cancer‐associated deletion at 10q23.3 corresponds to ∼2.06 MB region flanked by BMPR1A and FAS, which suggests a strong selective growth advantage for loss of PTEN and upregulation of PI3K/AKT can lead to recurrent microdeletions of the PTEN gene in prostate cancer.
Journal ArticleDOI
Aberrant promoter methylation and inactivation of PTEN gene in cervical carcinoma from Indian population
Meher Rizvi,M. Shabbir Alam,M. Shabbir Alam,Asgar Ali,Syed Jafar Mehdi,Swaraj Batra,Ashish K Mandal +6 more
TL;DR: Promoter methylation and loss of PTEN expression occur frequently in carcinoma of uterine cervix, suggesting that PTEN plays an important role in the carcinogenesis of cervical cancer.
Journal ArticleDOI
Mutation analysis of tumor suppressor gene PTEN in patients with gastric carcinomas and its impact on PI3K/AKT pathway
TL;DR: The results implicated that the mutations of PTEN did not occur at a significant rate in gastric carcinoma in Shanghai, but might play a role in tumorigenesis, hinting that PTEN might be a prognostic biomarker of gastric cancer.
References
More filters
Journal ArticleDOI
PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer
Jing Li,Clifford Yen,Danny Liaw,Katrina Podsypanina,Shikha Bose,Steven I. Wang,Janusz Puc,Christa Miliaresis,Linda Rodgers,Richard W. McCombie,Sandra H. Bigner,Beppino C. Giovanella,Michael Ittmann,B. Tycko,Hanina Hibshoosh,Michael Wigler,Ramon Parsons +16 more
TL;DR: The PTEN product has a protein tyrosine phosphatase domain and extensive homology to tensin, a protein that interacts with actin filaments at focal adhesions as discussed by the authors.
Journal ArticleDOI
Identification of a candidate tumour suppressor gene, MMAC1 , at chromosome 10q23.3 that is mutated in multiple advanced cancers
Peter A. Steck,Mark A. Pershouse,Samar A. Jasser,W. K. A. Yung,Huai Lin,Azra H. Ligon,Lauren A. Langford,Michelle Baumgard,T. Hattier,Thaylon Davis,Cheryl Frye,Rong Hu,Bradley D. Swedlund,David H. F. Teng,Sean V. Tavtigian +14 more
TL;DR: The results identify a strong candidate tumour suppressor gene at chromosome 10q23.3, whose loss of function appears to be associated with the oncogenesis of multiple human cancers.
Journal ArticleDOI
PTEN and myotubularin: novel phosphoinositide phosphatases.
TL;DR: Biochemical studies of the PTEN phosphatase have revealed a molecular mechanism by which tumorigenesis may be caused in individuals with PTEN mutations.
Journal ArticleDOI
Regulation of Cell Migration by the C2 Domain of the Tumor Suppressor PTEN
TL;DR: It is shown here that PTEN can also inhibit cell migration through its C2 domain, independent of its lipid phosphatase activity.
Journal ArticleDOI
Epstein-barr virus-positive gastric carcinoma demonstrates frequent aberrant methylation of multiple genes and constitutes CpG island methylator phenotype-positive gastric carcinoma
TL;DR: A relationship between EBV and aberrant methylation in GC is demonstrated and suggestions that aberrantmethylation may be an important mechanism of EBV-related gastric carcinogenesis are suggested.