Journal ArticleDOI
PCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and its Significance in Human Breast Cancer in Turkish Populations
TLDR
The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTENprotein expression was 68% in breast cancer tissue, which was significantly lower than that in paracancerous tissues (P < 0.005).Abstract:
The aim of this study was to analyze the possible effect of PTEN gene mutations on the occurrence and development of human breast cancer. PCR-SSCP-DNA sequencing indicated that two kinds of mutation sites were found in 4 out of 80 breast cancer samples. One kind of mutation was found in exons. AA-TCC mutation was located 40 bp upstream of the 3'lateral exon 2 (115946 AA-TCC). Such mutations led to terminator formation at codon 267 of the PTEN gene. The other mutation was found in an intron, including a C-T point mutation 91 bp upstream of 2' lateral exon 2 (1903858 C-T). The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTEN protein expression was 68% in breast cancer tissue, which was significantly lower than that (100%) in paracancerous tissues (P < 0.005). PTEN gene mutation may play an important role in the occurrence and development of breast cancer.read more
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Interaction of L-myc oncogene in breast cancer with irinotecan onto functionalized magnetic nanoparticles
TL;DR: In this article, the effect of different functional groups on binding capacity of DNA was studied with bare and modified superparamagnetic iron oxide nanoparticles (SPIONs), where modifications were performed with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane (GPTS) and (3-aminopropyl) triethoxysilane (APTS) by silanization reaction and also subsequent reaction with glutaraldehyde (GA) to obtain functional groups.
Journal ArticleDOI
Design of a probe based on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues
TL;DR: A new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues and showed good selectivity toward target DNA sequence.
References
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Journal ArticleDOI
PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer
Jing Li,Clifford Yen,Danny Liaw,Katrina Podsypanina,Shikha Bose,Steven I. Wang,Janusz Puc,Christa Miliaresis,Linda Rodgers,Richard W. McCombie,Sandra H. Bigner,Beppino C. Giovanella,Michael Ittmann,B. Tycko,Hanina Hibshoosh,Michael Wigler,Ramon Parsons +16 more
TL;DR: The PTEN product has a protein tyrosine phosphatase domain and extensive homology to tensin, a protein that interacts with actin filaments at focal adhesions as discussed by the authors.
Journal ArticleDOI
Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome
Danny Liaw,Deborah J. Marsh,Jing Li,Patricia L. M. Dahia,Steven I. Wang,Z. Zheng,Shikha Bose,K. M. Call,Hui C. Tsou,Monica Peacocke,Charis Eng,Ramon Parsons +11 more
TL;DR: Mutational analysis of PTEN in CD kindreds has identified germline mutations that are predicted to disrupt the protein tyrosine/dual-specificity phosphatase domain of this gene, and implies that PTEN may play a role in organizing the relationship of different cell types within an organ during development.
Journal ArticleDOI
Localization of the gene for Cowden disease to chromosome 10q22-23
Marcel R. Nelen,George W. Padberg,E. A. J. Peeters,Albert Y. Lin,B. Van Den Helm,Rune R. Frants,Valérie Coulon,Alisa M. Goldstein,M.M.M. van Reen,Douglas F. Easton,Rosalind A. Eeles,Shirley Hodgson,John J. Mulvihill,Victoria Murday,Monica Tucker,E.C.M. Mariman,T. M. Starink,Bruce Ponder,Hans-Hilger Ropers,Hannie Kremer,Michel Longy,Charis Eng,Charis Eng +22 more
TL;DR: Cowden disease (CD) (MIM 158350), or multiple hamartoma syndrome, is a rare autosomal dominant familial cancer syndrome with a high risk of breast cancer and central nervous system manifestations of CD were emphasized only recently.
Journal ArticleDOI
Mutational Spectra of PTEN/MMAC1 Gene: a Tumor Suppressor With Lipid Phosphatase Activity
TL;DR: The mutational spectra of the PTEN/MMAC1 gene in tumors from various tissues, especially endometrium, brain, prostate, and ovary, are reviewed, suggesting that depending on the tissue type, the gene appears to be involved in the initiation or the progression of cancers.
Journal Article
PTEN/MMAC1 Mutations in Endometrial Cancers
TL;DR: Data indicate that PTEN/MMAC1 is more commonly mutated than any other known gene in endometrial cancers, and may be responsible for several familial neoplastic disorders.