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Journal ArticleDOI

Phosphorylation of membrane proteins from Plasmodium berghei-infected red cells.

TLDR
In this paper, a phosphorylated membrane protein of apparent molecular weight 42,000, absent in membrane from normal cells, can be detected in membrane of infected cells, but not by triton X-100, indicating that it may be red cell actin.
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This article is published in Biochemical and Biophysical Research Communications.The article was published on 1979-04-13. It has received 14 citations till now. The article focuses on the topics: Vesicle-associated membrane protein 8 & Membrane protein.

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Citations
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Journal ArticleDOI

Membrane structure and function of malaria parasites and the infected erythrocyte.

TL;DR: The synchronous rupture of the red cell and merozoite release is marked by the periodic fever–chill cycles so characteristic of the malarial infection.
Journal ArticleDOI

Alterations in the surface membrane of red blood cells during malaria.

TL;DR: The identification of alterations in the surface components of malaria-infected erythrocytes which might constitute targets for host-protective (i.e., parasiticidal) immune responses is described in detail.
Journal ArticleDOI

A cytoplasmic requirement of red cells for invasion by malarial parasites

TL;DR: The results suggest that ATP is required for maintaining the turnover of phosphoryl groups of membrane-associated proteins, such as spectrin, and a basic scheme for the mechanism of the invasion process is suggested.
Journal ArticleDOI

Acidic phosphoproteins associated with the host erythrocyte membrane of erythrocytes infected with Plasmodium berghei and P. chabaudi

TL;DR: Immunofluorescence using the antibodies in combination with subcellular fractionation studies clearly shows that these phosphoproteins are associated with the host erythrocyte membrane and not the parasite.
Journal ArticleDOI

Membrane-associated phosphoproteins in Plasmodium berghei-infected murine erythrocytes.

TL;DR: Two-dimensional gel electrophoresis indicates that pp45 and pp68 are of parasite origin, and partial or complete proteolytic digestion reveals thatpp45 is phosphorylated at similar amino acid residues both in intact cells and in isolated membranes.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI

The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes

TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
Journal ArticleDOI

Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents

TL;DR: Treatment of isolated human erythrocyte membranes with Triton X-100 at ionic strength ⋍0.04 preferentially released all the glycerolipid and glycoprotein species and the polypeptide elution profile obtained with nonionic detergents is nearly reciprocal to that previously seen with a variety of agents which perturb proteins.
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