piRNAs initiate transcriptional silencing of spermatogenic genes during C. elegans germline development
Reads0
Chats0
TLDR
In this paper , the authors show that C. elegans piRNAs trigger the transcriptional silencing of hundreds of spermatogenic genes during sperminogenesis, promoting sperm differentiation and function.About:
This article is published in Developmental Cell.The article was published on 2022-01-01 and is currently open access. It has received 18 citations till now. The article focuses on the topics: Piwi-interacting RNA & Biology.read more
Citations
More filters
Journal ArticleDOI
FISH-quant v2: a scalable and modular tool for smFISH image analysis
Arthur Imbert,Wei Ouyang,Adham Safieddine,Emeline Coleno,Christophe Zimmer,Edouard Bertrand,Thomas Walter,Florian Mueller +7 more
TL;DR: FISH-quant v2, a highly modular tool accessible for both experts and non-experts, was validated and applied on large-scale smFISH image data sets, revealing diverse subcellular RNA localization patterns and a surprisingly high degree of cell-to-cell heterogeneity.
Journal ArticleDOI
Emerging roles and functional mechanisms of PIWI-interacting RNAs
TL;DR: The most recent advances in the understanding of piRNA biogenesis, the molecular mechanisms of pi RNA function and the emerging roles of piRNAs in germ line development mainly in flies and mice, and in infertility, cancer and neurological diseases in humans are discussed.
Journal ArticleDOI
Non-Coding RNAs as Novel Regulators of Neuroinflammation in Alzheimer’s Disease
TL;DR: In this article , the role of different types of ncRNAs in neuroinflammation during AD are summarized in order to improve our understanding of AD etiology and aid in the translation of basic research into clinical practice.
Posted ContentDOI
A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
Uri Seroussi,Andrew Lugowski,Lina Wadi,Robert X Lao,Alexandra R. Willis,Winnie Zhao,Adam E. Sundby,Amanda G. Charlesworth,Aaron W. Reinke,Julie M. Claycomb +9 more
TL;DR: This work systematically analyzed every C. elegans AGO, using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags to identify small RNA binding complements and determine the effects of ago loss on small RNA populations and developmental phenotypes.
Journal ArticleDOI
A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
TL;DR: In this paper , the authors systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags and determined the effects of ago loss on small RNA populations and developmental phenotypes.
References
More filters
Journal ArticleDOI
deepTools2: a next generation web server for deep-sequencing data analysis
Fidel Ramírez,Devon Ryan,Björn Grüning,Vivek Bhardwaj,Fabian Kilpert,Andreas S. Richter,Steffen Heyne,Friederike Dündar,Thomas Manke +8 more
TL;DR: An update to the Galaxy-based web server deepTools, which allows users to perform complete bioinformatic workflows ranging from quality controls and normalizations of aligned reads to integrative analyses, including clustering and visualization approaches, is presented.
Journal ArticleDOI
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
TL;DR: A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.
Journal ArticleDOI
A pyramid approach to subpixel registration based on intensity
TL;DR: An automatic subpixel registration algorithm that minimizes the mean square intensity difference between a reference and a test data set, which can be either images (two-dimensional) or volumes (three-dimensional).
Journal ArticleDOI
Snakemake--a scalable bioinformatics workflow engine.
Johannes Köster,Sven Rahmann +1 more
TL;DR: Snakemake is a workflow engine that provides a readable Python-based workflow definition language and a powerful execution environment that scales from single-core workstations to compute clusters without modifying the workflow.
Related Papers (5)
Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans
Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline
Partha Pratim Das,Partha Pratim Das,Partha Pratim Das,Marloes P. Bagijn,Marloes P. Bagijn,Leonard D. Goldstein,Julie R. Woolford,Julie R. Woolford,Nicolas J. Lehrbach,Nicolas J. Lehrbach,Alexandra Sapetschnig,Alexandra Sapetschnig,Heeran R. Buhecha,Heeran R. Buhecha,Michael J. Gilchrist,Kevin L. Howe,Rory Stark,Nik Matthews,Eugene Berezikov,René F. Ketting,Simon Tavaré,Eric A. Miska,Eric A. Miska +22 more