Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline
Partha Pratim Das,Partha Pratim Das,Partha Pratim Das,Marloes P. Bagijn,Marloes P. Bagijn,Leonard D. Goldstein,Julie R. Woolford,Julie R. Woolford,Nicolas J. Lehrbach,Nicolas J. Lehrbach,Alexandra Sapetschnig,Alexandra Sapetschnig,Heeran R. Buhecha,Heeran R. Buhecha,Michael J. Gilchrist,Kevin L. Howe,Rory Stark,Nik Matthews,Eugene Berezikov,René F. Ketting,Simon Tavaré,Eric A. Miska,Eric A. Miska +22 more
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TLDR
It is demonstrated that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing, which might suggest a link between piRNA and siRNA function.About:
This article is published in Molecular Cell.The article was published on 2008-07-11 and is currently open access. It has received 422 citations till now. The article focuses on the topics: RasiRNA & Piwi-interacting RNA.read more
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Small silencing RNAs: an expanding universe
TL;DR: Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs.
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The promises and pitfalls of RNA-interference-based therapeutics
Daniela Castanotto,John J. Rossi +1 more
TL;DR: The discovery that gene expression can be controlled by the Watson–Crick base-pairing of small RNAs with messenger RNAs containing complementary sequence — a process known as RNA interference — has markedly advanced the understanding of eukaryotic gene regulation and function.
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RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond
TL;DR: A growing number of functions are emerging for RNA interference in the nucleus, in addition to well-characterized roles in post-transcriptional gene silencing in the cytoplasm, and increasing evidence indicates that RNAi regulates transcription through interaction with transcriptional machinery.
Journal ArticleDOI
RNA-mediated epigenetic regulation of gene expression
Daniel Holoch,Danesh Moazed +1 more
TL;DR: These co-transcriptional silencing mechanisms form powerful RNA surveillance systems that detect and silence inappropriate transcription events, and provide a memory of these events via self-reinforcing epigenetic loops.
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Small RNAs as Guardians of the Genome
TL;DR: The conserved roles played by small RNAs in the adaptation of eukaryotes to coexist with their genomic colonists are reviewed.
References
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Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Journal ArticleDOI
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
TL;DR: Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin- 4 regulates lin- 14 translation via an antisense RNA-RNA interaction.
Journal ArticleDOI
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
TL;DR: In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of the gene set.
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