Predicting drug sensitivity and resistance: profiling ABC transporter genes in cancer cells
Gergely Szakács,Jean Philippe Annereau,Samir Lababidi,Uma Shankavaram,Angela Arciello,Kimberly J. Bussey,William C. Reinhold,Yanping Guo,Gary D. Kruh,Mark Reimers,John N. Weinstein,Michael M. Gottesman +11 more
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TLDR
This work profiled mRNA expression of the 48 known human ABC transporters in 60 diverse cancer cell lines used by the National Cancer Institute and found and validated compounds whose activity is potentiated, rather than antagonized, by the MDR1 multidrug transporter.About:
This article is published in Cancer Cell.The article was published on 2004-08-01 and is currently open access. It has received 512 citations till now. The article focuses on the topics: ATP-binding cassette transporter & Cancer cell.read more
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Cancer drug resistance: an evolving paradigm
TL;DR: There are now unprecedented opportunities to understand and overcome drug resistance through the clinical assessment of rational therapeutic drug combinations and the use of predictive biomarkers to enable patient stratification.
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Targeting multidrug resistance in cancer
Gergely Szakács,Jill K. Paterson,Joseph A. Ludwig,Catherine Booth-Genthe,Michael M. Gottesman +4 more
TL;DR: Various approaches to combating multidrug-resistant cancer are described, including the development of drugs that engage, evade or exploit efflux by ABC transporters.
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The NCI60 human tumour cell line anticancer drug screen
TL;DR: The development, use and productivity of the NCI60 screen are reviewed, highlighting several outcomes that have contributed to advances in cancer chemotherapy.
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Drug Resistance in Cancer: An Overview
Genevieve Housman,Shannon Byler,Sarah Heerboth,Karolina Lapinska,Mckenna Longacre,Nicole A. Snyder,Sibaji Sarkar +6 more
TL;DR: The current knowledge of mechanisms that promote or enable drug resistance, such as drug inactivation, drug target alteration, drug efflux, DNA damage repair, cell death inhibition, and the epithelial-mesenchymal transition, as well as how inherent tumor cell heterogeneity plays a role in drug resistance are outlined.
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Anatomical Profiling of Nuclear Receptor Expression Reveals a Hierarchical Transcriptional Network
Angie L. Bookout,Yangsik Jeong,Michael Downes,Ruth T. Yu,Ronald M. Evans,David J. Mangelsdorf +5 more
TL;DR: These data reveal a hierarchical transcriptional circuitry that extends beyond individual tissues to form a meganetwork governing physiology on an organismal scale and distinguishes between reproduction, development, and growth and nutrient uptake, metabolism, and excretion.
References
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Journal ArticleDOI
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Jo Vandesompele,Katleen De Preter,Filip Pattyn,Bruce Poppe,Nadine Van Roy,Anne De Paepe,Franki Speleman +6 more
TL;DR: The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which opens up the possibility of studying the biological relevance of small expression differences.
Journal ArticleDOI
Multidrug resistance in cancer: role of ATP–dependent transporters
TL;DR: The ability to predict and circumvent drug resistance is likely to improve chemotherapy, and it has become apparent that resistance exists against every effective drug, even the authors' newest agents.
Journal ArticleDOI
Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line
Susan P.C. Cole,Gabu Bhardwaj,James H. Gerlach,J. E. Mackie,Caroline E. Grant,Kurt C. Almquist,Alistair J. Stewart,Ebba U. Kurz,A. M. V. Duncan,Roger G. Deeley +9 more
TL;DR: Reversion to drug sensitivity was associated with loss of gene amplification and a marked decrease in mRNA expression, and the mRNA encodes a member of the ATP-binding cassette transmembrane transporter superfamily.
Journal ArticleDOI
Systematic variation in gene expression patterns in human cancer cell lines.
Douglas T. Ross,Uwe Scherf,Michael B. Eisen,Charles M. Perou,Christian A. Rees,Paul T. Spellman,Vishwanath R. Iyer,Stefanie S. Jeffrey,Matt van de Rijn,Mark Waltham,Alexander Pergamenschikov,Jeffrey C. Lee,Deval A. Lashkari,Dari Shalon,Timothy G. Myers,John N. Weinstein,David Botstein,Patrick O. Brown +17 more
TL;DR: Using cDNA microarrays to explore the variation in expression of approximately 8,000 unique genes among the 60 cell lines used in the National Cancer Institute's screen for anti-cancer drugs provided a novel molecular characterization of this important group of human cell lines and their relationships to tumours in vivo.
Journal ArticleDOI
Resampling-Based Multiple Testing: Examples and Methods for p-Value Adjustment.
TL;DR: Resampling-Based Adjustments: Basic Concepts and Practical Applications.