Journal ArticleDOI
Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist
Stephen P. Eisenberg,Ronald Evans,William P. Arend,Evie L. Verderber,Michael T Brewer,Hannum Charles H,Robert C. Thompson +6 more
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TLDR
Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity and analysis of monocyte RNA indicates that the gene is transcriptionally regulated.Abstract:
Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.read more
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A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.
Nancy A. Thornberry,Herbert G. Bull,Jimmy R. Calaycay,Kevin T. Chapman,Andrew D. Howard,Matthew J. Kostura,Douglas K. Miller,Susan M. Molineaux,Jeffrey R. Weidner,John G. Aunins,Keith O. Elliston,Julia M. Ayala,Francesca J. Casano,Jayne Chin,Gloria J.-F. Ding,Linda A. Egger,Erin P. Gaffney,Guadalupe A. Limjuco,Oksana C. Palyha,S.M. Raju,Anna M. Rolando,J. Paul Salley,Ting-Ting Yamin,Terry D. Lee,John E. Shively,Malcolm MacCross,Richard A. Mumford,John A. Schmidt,Michael J. Tocci +28 more
TL;DR: Purification and cloning of the complementary DNA indicates that IL-lβ-converting enzyme is composed of two nonidentical subunits that are derived from a single proenzyme, possibly by autoproteolysis.
Journal ArticleDOI
Interleukin-1 and interleukin-1 antagonism.
TL;DR: The recent cloning of a naturally occurring IL-1 receptor antagonist (IL-1ra) has opened new experimental and clinical approaches and reduced the severity of diseases such as hemodynamic shock, lethal sepsis, inflammatory bowel disease, experimental arthritis, and the spontaneous proliferation of human leukemic cells.
Journal ArticleDOI
Regulation of the Hypothalamic-Pituitary-Adrenal Axis by Cytokines: Actions and Mechanisms of Action
TL;DR: Findings are reviewed that have documented which cytokines have been shown to influence hormone secretion from the HPA axis, determined under what physiological/pathophysiological circumstances endogenous cytokines regulate HPAaxis activity, established the possible sites of cytokine action on HPA Axis hormone secretion, and identified the potential neuroanatomic and pharmacological mechanisms by which cytokine signal the neuroendocrine hypothalamus.
Journal ArticleDOI
Cytokines for psychologists: implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition.
Steven F. Maier,Linda R. Watkins +1 more
TL;DR: It is argued that activation of immune-brain pathways is important for understanding diverse phenomena related to stress such as depression and suppression of specific immunity.
Journal ArticleDOI
Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor
Hannum Charles H,Carol J. Wilcox,William P. Arend,Joslin Fenneke G,David Dripps,Patricia L. Heimdal,Lyman G Armes,Andreas Sommer,Stephen P. Eisenberg,Robert C. Thompson +9 more
TL;DR: Three interleukin-1 inhibitors have been purified to homogeneity from medium conditioned by human monocytes and partial sequence analysis and digestion with N-glycanase indicate that these are glycosylation forms of a single protein.
References
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Journal ArticleDOI
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Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
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DNA sequencing with chain-terminating inhibitors
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Journal ArticleDOI
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TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.