Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC.
Juraj Lenčo,Martin Hubálek,Pär Larsson,Alena Fučíková,Martin Brychta,Ales Macela,Jiří Stulík +6 more
TLDR
Results, obtained at the proteome level, support and confirm recently published data showing that the igl operon genes are transcribed in response to iron limitation, and help clarify the virulence mechanisms of F. tularensis.Abstract:
Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In this study, the response of F. tularensis live vaccine strain to iron depletion, which simulates the environment within the host, was investigated. In order to detect alterations in protein synthesis, metabolic labeling, followed by 2D-PAGE analysis was used. Globally, 141 protein spots were detected whose levels were significantly altered in the iron-restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at an increased level during iron-limited growth, three proteins were found encoded by the igl operon, located in the F. tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full virulence of F. tularensis. These results, obtained at the proteome level, support and confirm recently published data showing that the igl operon genes are transcribed in response to iron limitation.read more
Citations
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Nooks and Crannies in Type VI Secretion Regulation
TL;DR: Type VI secretion systems (T6SS) are macromolecular, transenvelope machines encoded within the genomes of most Gram-negative bacteria, including plant, animal, and human pathogens, as well as soil and environmental isolates, and how they have been integrated into existing regulatory networks is highlighted.
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The Role of the Francisella Tularensis Pathogenicity Island in Type VI Secretion, Intracellular Survival, and Modulation of Host Cell Signaling.
TL;DR: The current knowledge regarding the components of the FPI and the roles that have been ascribed to them are described and described.
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Working toward the Future: Insights into Francisella tularensis Pathogenesis and Vaccine Development
TL;DR: The major properties of several Francisella species, including F. tularensis and F. novicida, are summarized and an up-to-date synopsis of the genes necessary for pathogenesis by these organisms and the determinants that are currently being targeted for vaccine development is provided.
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Identification of fevR, a Novel Regulator of Virulence Gene Expression in Francisella novicida
Anna Brotcke,Denise M. Monack +1 more
TL;DR: FevR is a crucial virulence gene in Francisella, required for the expression of virulence factors known to be essential for this pathogen's subversion of host defenses and pathogenesis in vivo.
Journal ArticleDOI
Molecular and genetic basis of pathogenesis in Francisella tularensis
Jeffrey R. Barker,Karl E. Klose +1 more
TL;DR: A virulence factor, MglA, has been identified that is essential for this ability of F. tularensis to survive and replicate within macrophages, and a number of virulence genes identified through transposon mutant screens are identified.
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